Mechanism of Assembly of the Tyrosyl Radical-Diiron(III) Cofactor of E. coli Ribonucleotide Reductase. 3. Kinetics of the Limiting Fe2+Reaction by Optical, EPR, and Mossbauer Spectroscopies

J. Martin Bollinger, Wing Hang Tong, Jo Anne Stubbe, Boi Hann Huynh, Dale E. Edmondson, Natarajan Ravi

Research output: Contribution to journalArticle

131 Scopus citations

Abstract

The R2 subunit of E. coli ribonucleotide reductase contains a tyrosyl radical-diiron(III) cofactor which assembles spontaneously when apo R2 is mixed with Fe2+and O2. The kinetic/spectroscopic characteristics of this assembly reaction were previously shown to depend on the Fe2+/apo R2 ratio (Bollinger, J. M., Jr. et al. Science 1991, 253,292-298). In the case of the reaction carried out with limiting Fe2+(Fe2+/apo R2 = 2.0-2.4), two intermediates were proposed to accumulate. On the basis of its broad absorption feature at 560 nm, the first intermediate was suggested to contain a μ-peroxodiferric cluster. The one-electron oxidation of tyrosine 122 by this cluster was postulated to generate the second intermediate, which was proposed to contain the tyrosyl radical (∗Y122) and the diferric radical species (Ravi, N. et al. J. Am. Chem. Soc, first of three papers in this issue; Bollinger, J. M., Jr. et al. J. Am. Chem. Soc. 1991,113,6289-6291). In this study, stopped-flow absorption, rapid freeze-quench electron paramagnetic resonance, and rapid freeze-quench Mössbauer spectroscopies have been used to characterize in detail the kinetics of the assembly reaction carried out with limiting Fe2+. The time course of development and decay of the 560 nm absorption transient, and the quantities of diferric radical species, ∗Y122, and product diferric cluster as functions of time, are consistent with the proposal that the 560 nm absorbing species generates ∗Y122. The data indicate, in contrast to our previous hypothesis, that the 560 nm absorbing species is not iron based. It is proposed, instead, that the species is a tryptophan radical cation produced by the one-electron oxidation of W48. The results of this study are consistent with our previous proposal that Fe(IV) intermediates are not involved in generation of ∗Y122.

Original languageEnglish (US)
Pages (from-to)8024-8032
Number of pages9
JournalJournal of the American Chemical Society
Volume116
Issue number18
DOIs
StatePublished - Sep 1 1994

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this