Mechanism of DNA polymerase I: Exonuclease/polymerase activity and DNA sequence dependence of pyrophosphorolysis and misincorporation reactions

V. Mizrahi, P. Benkovic, S. J. Benkovic

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Mechanistic features of several processes involved in the idling-turnover reaction catalyzed by the large (Klenow) fragment of Escherichia coli DNA polymerase I have been established. The exonuclease → polymerase activity switch involved in the excision/incorporation mode of idling-turnover occurs without an intervening dissociation of the enzyme from its DNA substrate. Comparative studies on the pyrophosphorolysis kinetics of related DNA substrates indicate a significant dependence of the reaction rate upon the DNA sequence within the duplex region upstream of the primer-template junction. Finally, a gel electrophoretic analysis of the products of the idling-turnover reaction has provided direct evidence for an alternative DNA sequence-dependent misincorporation/excision pathway.

Original languageEnglish (US)
Pages (from-to)5769-5773
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number16
DOIs
StatePublished - 1986

All Science Journal Classification (ASJC) codes

  • General

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