Mechanism of Enzyme Regulation by Calmodulin and Ca2+

Luisa Gregori, Patrick M. Gillevet, Paul Doan, Vincent Chau

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

This chapter discusses the mechanism of enzyme regulation by calmodulin and Ca2+. The interactions between Ca2+, calmodulin, and a calmodulin-dependent enzyme can be described by a set of macroscopic equilibria. Information on the various interactions between Ca2+, calmodulin, and calmodulin-dependent enzymes is of particular importance as these interactions ultimately dictate the nature of the Ca2+ dependence of these enzymes. Quantitative characterization of these interactions has been hindered by the fact that the interaction between calmodulin and its target enzymes in the absence of Ca2+ is usually of very low affinity, and the Ca2+ binding to the enzyme-calmodulin complex cannot be determined directly. The activity of PDE as a function of Ca2+ and calmodulin can be measured with high precision by using the technique of flow-microcalorimetry to monitor the PDE-catalyzed hydrolysis of cAMP. A possible structural change on calmodulin when it binds to PDE is the reorientation of the α-helices flanking the Ca2+-binding loop. This would lead to a change in the position of the amino acid residues that coordinate with Ca2+ and thus change the affinity of Ca2+. Recent NMR studies indicate that the positions of the α-helices in calmodulin are altered when the protein is bound with Ca2+; whether the helices undergo further reorientation with enzyme binding remains to be determined.

Original languageEnglish (US)
Title of host publicationCurrent Topics in Cellular Regulation
Pages447-454
Number of pages8
EditionC
DOIs
StatePublished - Jan 1 1985

Publication series

NameCurrent Topics in Cellular Regulation
NumberC
Volume27
ISSN (Print)0070-2137

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Gregori, L., Gillevet, P. M., Doan, P., & Chau, V. (1985). Mechanism of Enzyme Regulation by Calmodulin and Ca2+ In Current Topics in Cellular Regulation (C ed., pp. 447-454). (Current Topics in Cellular Regulation; Vol. 27, No. C). https://doi.org/10.1016/B978-0-12-152827-0.50045-1