Mechanism of transcriptional activation by Sp1: Evidence for coactivators

B. Franklin Pugh, Robert Tjian

Research output: Contribution to journalArticlepeer-review

726 Scopus citations

Abstract

In reconstituted reactions, Sp1 stimulates transcription at TATA-containing promoters in the presence of semipurified TFIID fractions from either human or Drosophila cells, but is unable to do so when these fractions are replaced by purified, cloned Drosophila or yeast TFIID. Our findings with Sp1 and CTF suggest that partially purified TFIID fractions from human and Drosophila cells contain coactivators that are dispensable for basal transcription but are required as molecular adaptors between trans-activators and the general transcription initiation machinery. Experiments using cloned TFIID proteins suggest that these coactivators function through the amino-terminal portion of TFIID and that coactivator-TFIID interactions are species specific. At promoters lacking a TATA box, an additional activity distinct from coactivators is required for Sp1 activation of transcription.

Original languageEnglish (US)
Pages (from-to)1187-1197
Number of pages11
JournalCell
Volume61
Issue number7
DOIs
StatePublished - Jun 29 1990

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Mechanism of transcriptional activation by Sp1: Evidence for coactivators'. Together they form a unique fingerprint.

Cite this