Mechanisms of activation and suppression in rat Nb 2 lymphoma cells: A model for interactions between prolactin and the immune system

Lisa G. Bates, Deborah S. Grove, Andrea Marie Mastro

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Rat Nb 2 lymphoma cells have been widely used to bioassay human growth hormone and many species of prolactin. Because their morphologic characterization suggests a T-cell lineage, Nb 2 cells were examined for their response to the T-cell mitogens concanavalin A, pokeweed mitogen, and phytohemagglutinin P. As expected, a dose-response to rat prolactin was observed; however, attempts to induce proliferation using the conventional T- cell mitogens failed at concentrations normally stimulatory for rat primary lymphocytes. Moreover, when Nb 2 cells were simultaneously incubated with lectin plus a suboptimal concentration of prolactin, a dose-dependent suppression of the stimulatory effects of prolactin was observed with phytohemagglutinin P and pokeweed mitogen, although not with concanavalin A. Culture medium of prolactin-stimulated Nb 2 cells also contained a factor which inhibited normal rat lymphocyte activation by concanavalin A. The factor did not block induction of the IL-2 receptor and proliferation of IL- 2-dependent CTLL-2 cells could be restored by exogenous IL-2. Because Nb 2 cells evolved from a lactogen-dependent lymph node tumor, these results may have implications for further understanding the role of pituitary hormones, particularly prolactin, in the immune response to hormone-dependent tumor progression.

Original languageEnglish (US)
Pages (from-to)567-572
Number of pages6
JournalExperimental Cell Research
Volume218
Issue number2
DOIs
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Cell Biology

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