Mechanisms of herpes simplex virus infectivity enhanced by ultracentrifugal inoculation

Ultracentrifugation of very dilute suspensions of herpes simplex virus directly onto monolayer cells grown in centrifuge tubes was studied. Enhanced infectivity by ultracentrifugation was similar at 4°C and at 35 to 37°C. The high infectivity levels of cultures centrifuged at 4°C were further examined by infectious center assays. At 4°C, the numbers of infectious centers in control (noncentrifuged) cultures were almost 100-fold fewer than in control cultures at 37°C. However, the numbers of infectious centers in cultures ultracentrifuged at 4°C were similar to those ultracentrifuged at 37°C. The great difference in the numbers of infectious centers between 4 and 37°C control cultures, in contrast to the similarity between 4 and 37°C ultracentrifuged cultures, indicated that ultracentrifugation at 4°C enhanced infectivity possibly by facilitation of herpes simplex virus penetration into monolayer cells.