Mechanisms of resistance to three mite growth inhibitors of Tetranychus urticae in hops

A. W. Adesanya, M. A. Morales, D. B. Walsh, L. C. Lavine, M. D. Lavine, F. Zhu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mite growth inhibitors (MGIs), such as etoxazole and hexythiazox, are valuable IPM tools for Tetranychus urticae control in hops due to their unique mode of action and selectivity. Hence, it is necessary to standardize bioassay methods to evaluate the efficacy of MGIs, monitor resistance, and identify mechanisms underlying MGI resistance in the field. Here, we developed a three-tiered approach for evaluating ovicidal toxicity of MGIs to T. urticae, which simulated different MGI exposure scenarios in the field. The most effective bioassay method was direct exposure of T. urticae eggs to MGIs. With this method, four field-collected T. urticae populations showed low-to-moderate resistance to MGIs. Cross-resistance among MGIs and from MGIs to bifenazate and bifenthrin was detected. Besides target site insensitivity, enhanced cytochrome P450 and esterase activities also contribute to the MGI resistance in hop yard-collected T. urticae populations. Low-to-moderate MGI resistance in T. urticae populations may be mediated by multiple mechanisms. Positive selection pressure on the I1017F mutation is moderate in field-collected T. urticae populations. Further studies are required to identify metabolic detoxification genes that confer resistance to MGIs for precise resistance monitoring.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalBulletin of Entomological Research
DOIs
StateAccepted/In press - May 3 2017

Fingerprint

hops
Tetranychus urticae
resistance mechanisms
mites
hexythiazox
bioassays
metabolic detoxification
exposure scenario
bifenthrin
monitoring
cross resistance
direct contact
cytochrome P-450
esterases
mechanism of action
methodology
toxicity

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Insect Science

Cite this

Adesanya, A. W. ; Morales, M. A. ; Walsh, D. B. ; Lavine, L. C. ; Lavine, M. D. ; Zhu, F. / Mechanisms of resistance to three mite growth inhibitors of Tetranychus urticae in hops. In: Bulletin of Entomological Research. 2017 ; pp. 1-12.
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abstract = "Mite growth inhibitors (MGIs), such as etoxazole and hexythiazox, are valuable IPM tools for Tetranychus urticae control in hops due to their unique mode of action and selectivity. Hence, it is necessary to standardize bioassay methods to evaluate the efficacy of MGIs, monitor resistance, and identify mechanisms underlying MGI resistance in the field. Here, we developed a three-tiered approach for evaluating ovicidal toxicity of MGIs to T. urticae, which simulated different MGI exposure scenarios in the field. The most effective bioassay method was direct exposure of T. urticae eggs to MGIs. With this method, four field-collected T. urticae populations showed low-to-moderate resistance to MGIs. Cross-resistance among MGIs and from MGIs to bifenazate and bifenthrin was detected. Besides target site insensitivity, enhanced cytochrome P450 and esterase activities also contribute to the MGI resistance in hop yard-collected T. urticae populations. Low-to-moderate MGI resistance in T. urticae populations may be mediated by multiple mechanisms. Positive selection pressure on the I1017F mutation is moderate in field-collected T. urticae populations. Further studies are required to identify metabolic detoxification genes that confer resistance to MGIs for precise resistance monitoring.",
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Mechanisms of resistance to three mite growth inhibitors of Tetranychus urticae in hops. / Adesanya, A. W.; Morales, M. A.; Walsh, D. B.; Lavine, L. C.; Lavine, M. D.; Zhu, F.

In: Bulletin of Entomological Research, 03.05.2017, p. 1-12.

Research output: Contribution to journalArticle

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AU - Morales, M. A.

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