Mediators of interleukin-1 beta action on the Na+-K +ATPase in Caco-2 cells

Rana Al-Sadi, Sawsan Ibrahim Kreydiyyeh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Interleukin1-β has been demonstrated previously to reduce the activity and expression of the Na+-K+ pump in the rat jejunum and colon. This work attempts to elucidate the signal transduction pathway underlying its effect using Caco-2 cells. IL-1β reduced, in these cells also, the activity and expression of ATPase, in a dose- and time-dependent manner. The down-regulatory effect of the cytokine on the ATPase was not evident, when p38 MAP kinase was inhibited, but appeared in presence of inhibitors of MEK and NFκB, although activation of NF-κB was demonstrated by western blot analysis. The effect of IL-1β on the pump disappeared in the presence of indomethacin, a COX inhibitor. Exogenous PGE2 reduced the expression of the pump within 15 minutes, and this effect was still apparent when p38MAPK was inhibited. Curcumin, a JNK/AP-1 inhibitor, partially abolished the effect of IL-1β on ATPase expression but did not interfere with the effect of PGE2. These results indicate that IL-1β reduces the expression of ATPase independently of NFκB but, through a major pathway involving p38 and COX-2/PGE2, and another pathway involving JNK/AP1.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalEuropean Cytokine Network
Volume14
Issue number2
StatePublished - Apr 1 2003

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Caco-2 Cells
Interleukin-1beta
Interleukin-1
Adenosine Triphosphatases
Dinoprostone
Pumps
Signal transduction
Curcumin
MAP Kinase Signaling System
Mitogen-Activated Protein Kinase Kinases
Transcription Factor AP-1
p38 Mitogen-Activated Protein Kinases
Jejunum
Indomethacin
Rats
Signal Transduction
Colon
Western Blotting
Chemical activation
Cytokines

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry

Cite this

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abstract = "Interleukin1-β has been demonstrated previously to reduce the activity and expression of the Na+-K+ pump in the rat jejunum and colon. This work attempts to elucidate the signal transduction pathway underlying its effect using Caco-2 cells. IL-1β reduced, in these cells also, the activity and expression of ATPase, in a dose- and time-dependent manner. The down-regulatory effect of the cytokine on the ATPase was not evident, when p38 MAP kinase was inhibited, but appeared in presence of inhibitors of MEK and NFκB, although activation of NF-κB was demonstrated by western blot analysis. The effect of IL-1β on the pump disappeared in the presence of indomethacin, a COX inhibitor. Exogenous PGE2 reduced the expression of the pump within 15 minutes, and this effect was still apparent when p38MAPK was inhibited. Curcumin, a JNK/AP-1 inhibitor, partially abolished the effect of IL-1β on ATPase expression but did not interfere with the effect of PGE2. These results indicate that IL-1β reduces the expression of ATPase independently of NFκB but, through a major pathway involving p38 and COX-2/PGE2, and another pathway involving JNK/AP1.",
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Mediators of interleukin-1 beta action on the Na+-K +ATPase in Caco-2 cells. / Al-Sadi, Rana; Kreydiyyeh, Sawsan Ibrahim.

In: European Cytokine Network, Vol. 14, No. 2, 01.04.2003, p. 83-90.

Research output: Contribution to journalArticle

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