Medium evaluation and plastic composite support ingredient selection for biofilm formation and succinic acid production by Actinobacillus succinogenes

Susan E. Urbance, Anthony L. Pometto, Alan A. DiSpirito, Ali Demirci

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Succinic acid production medium for Actinobacillus succinogenes was simplified. Vitamins and fatty acids were removed from the medium and industrial quality yeast extract was substituted to yield revised succinic acid medium. Customized plastic composite support (PCS) blends were screened for biofilm and succinic acid production. Twenty different PCS blends with and without mineral salt additions were evaluated in twenty simulated repeated-batch fermentations using MgCO3 for pH control and CO2 supply. Succinic acid concentrations, percentage yield succinic acid, and biofilm formation for each PCS blend were determined. No correlation was observed for biofilm formation and succinic acid production. The selected customized PCS blend for A. succinogenes succinic acid production consisted of 50% (wt/wt) polypropylene, 35% (wt/wt) dried ground soybean hulls, 5% (wt/wt) dried bovine albumin, 2.5% (wt/wt) soybean flour, 2.5% (wt/wt) yeast extract, 2.5% (wt/wt) dried red blood cells, and 2.5% (wt/wt) peptone, which demonstrated 70% yields for succinic acid compared to 64% yield for suspended cell fermentation.

Original languageEnglish (US)
Pages (from-to)53-65
Number of pages13
JournalFood Biotechnology
Volume17
Issue number1
DOIs
StatePublished - Jun 19 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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