Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro

Anne W. Hamburger, Howard Parnes, Gary B. Gordon, Lisa Shantz, Kelly A. O'Donnell, Joseph Aisner

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We recently observed significant weight gain in patients with advanced breast cancer treated with high-dose megestrol acetate. However, the mechanisms of this effect are completely unknown. As the analysis of the action of steroids in vivo is complex, we chose to examine the effects of megestrol acetate on the differentiation of a preadipocyte clone (L1) of the Swiss 3T3 mouse fibroblast cell line. Addition of insulin, fetal bovine serum, dexamethasone, and methyl-isobutylaxanthine to confluent 3T3-L1 cells induced adipocyte differentiation, as verified by morphologic studies and analysis of the activity of the enzyme glycerol-3-phosphate dehydrogenase (G-3-PD), a specific and sensitive indicator of lipocyte function. Substitution of megestrol acetate for dexamethasone also resulted in greatly increased lipocyte differentiation. Tumor necrosis factor (TNF) α blocked the adipocyte differentiation induced by the combination of insulin, dexamethasone, and methyl-isobutylxanthine. Addition of megestrol acetate failed to reverse the inhibitory effect of TNF. Thus, megestrol acetate, in vitro, is a potent inducer of lipocyte differentiation. Further studies on the regulation of adipocyte differentiation and function in this model system may be important to clarify megestrol acetate's mechanism of action.

Original languageEnglish (US)
Pages (from-to)76-78
Number of pages3
JournalSeminars in oncology
Volume15
Issue number2 SUPPL. 1
StatePublished - Jan 1 1988

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Megestrol Acetate
Adipocytes
Dexamethasone
Tumor Necrosis Factor-alpha
Insulin
3T3-L1 Cells
Glycerolphosphate Dehydrogenase
Weight Gain
In Vitro Techniques
Clone Cells
Fibroblasts
Steroids
Breast Neoplasms
Cell Line
Enzymes
Serum

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology

Cite this

Hamburger, A. W., Parnes, H., Gordon, G. B., Shantz, L., O'Donnell, K. A., & Aisner, J. (1988). Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro. Seminars in oncology, 15(2 SUPPL. 1), 76-78.
Hamburger, Anne W. ; Parnes, Howard ; Gordon, Gary B. ; Shantz, Lisa ; O'Donnell, Kelly A. ; Aisner, Joseph. / Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro. In: Seminars in oncology. 1988 ; Vol. 15, No. 2 SUPPL. 1. pp. 76-78.
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Hamburger, AW, Parnes, H, Gordon, GB, Shantz, L, O'Donnell, KA & Aisner, J 1988, 'Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro', Seminars in oncology, vol. 15, no. 2 SUPPL. 1, pp. 76-78.

Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro. / Hamburger, Anne W.; Parnes, Howard; Gordon, Gary B.; Shantz, Lisa; O'Donnell, Kelly A.; Aisner, Joseph.

In: Seminars in oncology, Vol. 15, No. 2 SUPPL. 1, 01.01.1988, p. 76-78.

Research output: Contribution to journalArticle

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Hamburger AW, Parnes H, Gordon GB, Shantz L, O'Donnell KA, Aisner J. Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro. Seminars in oncology. 1988 Jan 1;15(2 SUPPL. 1):76-78.