Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro

Anne W. Hamburger, Howard Parnes, Gary B. Gordon, Lisa Shantz, Kelly A. O'Donnell, Joseph Aisner

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

We recently observed significant weight gain in patients with advanced breast cancer treated with high-dose megestrol acetate. However, the mechanisms of this effect are completely unknown. As the analysis of the action of steroids in vivo is complex, we chose to examine the effects of megestrol acetate on the differentiation of a preadipocyte clone (L1) of the Swiss 3T3 mouse fibroblast cell line. Addition of insulin, fetal bovine serum, dexamethasone, and methyl-isobutylaxanthine to confluent 3T3-L1 cells induced adipocyte differentiation, as verified by morphologic studies and analysis of the activity of the enzyme glycerol-3-phosphate dehydrogenase (G-3-PD), a specific and sensitive indicator of lipocyte function. Substitution of megestrol acetate for dexamethasone also resulted in greatly increased lipocyte differentiation. Tumor necrosis factor (TNF) α blocked the adipocyte differentiation induced by the combination of insulin, dexamethasone, and methyl-isobutylxanthine. Addition of megestrol acetate failed to reverse the inhibitory effect of TNF. Thus, megestrol acetate, in vitro, is a potent inducer of lipocyte differentiation. Further studies on the regulation of adipocyte differentiation and function in this model system may be important to clarify megestrol acetate's mechanism of action.

Original languageEnglish (US)
Pages (from-to)76-78
Number of pages3
JournalSeminars in oncology
Volume15
Issue number2 SUPPL. 1
StatePublished - Jan 1 1988

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology

Fingerprint Dive into the research topics of 'Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro'. Together they form a unique fingerprint.

  • Cite this

    Hamburger, A. W., Parnes, H., Gordon, G. B., Shantz, L., O'Donnell, K. A., & Aisner, J. (1988). Megestrol acetate-induced differentiation of 3T3-L1 adipocytes in vitro. Seminars in oncology, 15(2 SUPPL. 1), 76-78.