Membrane association and conformational change of palmitoylated Goα

Shengyu Yang, Ling Zhang, Youguo Huang

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Bovine brain Goα was specifically palmitoylated in vitro. The apparent dissociation constant for depalmitoylated Goα (dGoα) was 0.273 μM, while that for palmitoylated Goα (pGoα) was 5.77 nM. The dissociation rate constant (K21) and dissociation half-life for dGoα were 8.4 × 10-4 min and 825 min respectively, while no significant dissociation of pGoα was detected. The limiting membrane insertion pressures for pGoα and dGoα were 44.4 mN/m and 41.3 mN/m respectively. These data suggested that palmitoylation facilitated the membrane association of Goα. Conformational changes of dGoα and pGoα detected by monitoring fluorescence spectra and fluorescence quenching were significantly different after they were associated with the membrane. It was suggested that conformational changes of Goα upon membrane association might be related to regulation of Goα signaling by palmitoylation.

Original languageEnglish (US)
Pages (from-to)76-81
Number of pages6
JournalFEBS Letters
Volume498
Issue number1
DOIs
StatePublished - Jun 1 2001

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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