Membrane association of VP22, a herpes simplex virus type 1 tegument protein

Michael J. Brignati, Joshua S. Loomis, John W. Wills, Richard J. Courtney

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Tegument proteins of herpes simplex virus type 1 (HSV-1) are hypothesized to contain the functional information required for the budding or envelopment process proposed to occur at cytoplasmic compartments of the host cell. One of the most abundant tegument proteins of HSV-1 is the UL49 gene product, VP22, a 38-kDa protein of unknown function. To study its subcellular localization, a VP22-green fluorescent protein chimera was expressed in transfected human melanoma (A7) cells. In the absence of other HSV-1 proteins, VP22 localizes to acidic compartments of the cell that may include the trans-Golgi network (TGN), suggesting that this protein is membrane associated. Membrane pelleting and membrane flotation assays confirmed that VP22 partitions with the cellular membrane fraction. Through truncation mutagenesis, we determined that the membrane association of VP22 is a property attributed to amino acids 120 to 225 of this 301-amino-acid protein. The above results demonstrate that VP22 contains specific information required for targeting to membranes of acidic compartments of the cell which may be derived from the TGN, suggesting a potential role for VP22 during tegumentation and/or final envelopment.

Original languageEnglish (US)
Pages (from-to)4888-4898
Number of pages11
JournalJournal of virology
Volume77
Issue number8
DOIs
StatePublished - Apr 1 2003

Fingerprint

Human herpesvirus 1
Human Herpesvirus 1
Membranes
trans-Golgi Network
Proteins
proteins
Amino Acids
cells
Green Fluorescent Proteins
amino acids
Mutagenesis
chimerism
melanoma
Melanoma
green fluorescent protein
Membrane Proteins
mutagenesis
Genes
assays

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Brignati, Michael J. ; Loomis, Joshua S. ; Wills, John W. ; Courtney, Richard J. / Membrane association of VP22, a herpes simplex virus type 1 tegument protein. In: Journal of virology. 2003 ; Vol. 77, No. 8. pp. 4888-4898.
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Membrane association of VP22, a herpes simplex virus type 1 tegument protein. / Brignati, Michael J.; Loomis, Joshua S.; Wills, John W.; Courtney, Richard J.

In: Journal of virology, Vol. 77, No. 8, 01.04.2003, p. 4888-4898.

Research output: Contribution to journalArticle

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