Membrane raft disruption promotes axonogenesis in N2a neuroblastoma cells

Kimberly A. Petro, Cara Lynne Schengrund

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Membrane rafts are discrete microdomains found in cell membranes that contain cholesterol and glycosphingolipids such as gangliosides. As cholesterol is a major component of membrane rafts, its sequestration by the polyene filipin can be used to disrupt them. In previous work we observed that membrane raft disruption by filipin treatment of murine neuroblastoma N2a cells led to changes in expression of cell processes. In this study, we determined the type of process formation induced by filipin treatment as well as whether their expression was accompanied by changes in ganglioside content or subcellular distribution. The results indicate that the processes formed were axonal in nature and their expression was accompanied by changes in both ganglioside content as well as the subcellular localization of GM1.

Original languageEnglish (US)
Pages (from-to)29-37
Number of pages9
JournalNeurochemical Research
Volume34
Issue number1
DOIs
StatePublished - Jan 1 2009

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Filipin
Gangliosides
Neuroblastoma
Membranes
Cholesterol
Polyenes
Glycosphingolipids
Cell membranes
Cells
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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Membrane raft disruption promotes axonogenesis in N2a neuroblastoma cells. / Petro, Kimberly A.; Schengrund, Cara Lynne.

In: Neurochemical Research, Vol. 34, No. 1, 01.01.2009, p. 29-37.

Research output: Contribution to journalArticle

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