Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels

Rong Yin, Shawn J. Rice, Jinwei Wang, Lin Gao, Joseph Tsai, Radean T. Anvari, Fang Zhou, Xin Liu, Gang Wang, Yuxin Tang, Martin C.Mihm Jr, Chandra P. Belani, Dong Bao Chen, J. Stuart Nelson, Wenbin Tan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction. Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS. Methods. Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM). Results. 107 out of 299 identified proteins showed differential expressions in PWS lesions as compared to normal skin, mainly involving the functions of biosynthesis, membrane trafficking, cytoskeleton and cell adhesion/migration. The confirmative studies showed that expressions of membrane trafficking/ exocytosis related proteins such as VAT1, IQGAP1, HSC70, clathrin, perlecan, spectrin α1 and GDIR1 were significantly increased in PWS blood vessels as compared to normal ones. Furthermore, TEM studies showed there is a significant upregulation of extracellular vesicle exocytosis from PWS blood vessels as compared to control. Conclusions. The biological process of membrane trafficking and exocytosis is enhanced in PWS blood vessels. Our results imply that the extracellular vesicles released by lesional endothelial cells may act as potential intercellular signaling mediators to contribute to the pathogenesis of PWS.

Original languageEnglish (US)
Pages (from-to)479-490
Number of pages12
JournalHistology and histopathology
Volume34
Issue number5
DOIs
StatePublished - May 2019

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Port-Wine Stain
Exocytosis
Blood Vessels
Membranes
Transmission Electron Microscopy
Endothelial Cells
Biological Phenomena
Spectrin
Clathrin
Proteins
Venules
Cytoskeleton
Cell Adhesion
Cell Movement
Dilatation
Up-Regulation
Immunohistochemistry
Ions
Skin

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

Cite this

Yin, R., Rice, S. J., Wang, J., Gao, L., Tsai, J., Anvari, R. T., ... Tan, W. (2019). Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels. Histology and histopathology, 34(5), 479-490. https://doi.org/10.14670/HH-18-051
Yin, Rong ; Rice, Shawn J. ; Wang, Jinwei ; Gao, Lin ; Tsai, Joseph ; Anvari, Radean T. ; Zhou, Fang ; Liu, Xin ; Wang, Gang ; Tang, Yuxin ; Jr, Martin C.Mihm ; Belani, Chandra P. ; Chen, Dong Bao ; Nelson, J. Stuart ; Tan, Wenbin. / Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels. In: Histology and histopathology. 2019 ; Vol. 34, No. 5. pp. 479-490.
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abstract = "Introduction. Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS. Methods. Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM). Results. 107 out of 299 identified proteins showed differential expressions in PWS lesions as compared to normal skin, mainly involving the functions of biosynthesis, membrane trafficking, cytoskeleton and cell adhesion/migration. The confirmative studies showed that expressions of membrane trafficking/ exocytosis related proteins such as VAT1, IQGAP1, HSC70, clathrin, perlecan, spectrin α1 and GDIR1 were significantly increased in PWS blood vessels as compared to normal ones. Furthermore, TEM studies showed there is a significant upregulation of extracellular vesicle exocytosis from PWS blood vessels as compared to control. Conclusions. The biological process of membrane trafficking and exocytosis is enhanced in PWS blood vessels. Our results imply that the extracellular vesicles released by lesional endothelial cells may act as potential intercellular signaling mediators to contribute to the pathogenesis of PWS.",
author = "Rong Yin and Rice, {Shawn J.} and Jinwei Wang and Lin Gao and Joseph Tsai and Anvari, {Radean T.} and Fang Zhou and Xin Liu and Gang Wang and Yuxin Tang and Jr, {Martin C.Mihm} and Belani, {Chandra P.} and Chen, {Dong Bao} and Nelson, {J. Stuart} and Wenbin Tan",
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Yin, R, Rice, SJ, Wang, J, Gao, L, Tsai, J, Anvari, RT, Zhou, F, Liu, X, Wang, G, Tang, Y, Jr, MCM, Belani, CP, Chen, DB, Nelson, JS & Tan, W 2019, 'Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels', Histology and histopathology, vol. 34, no. 5, pp. 479-490. https://doi.org/10.14670/HH-18-051

Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels. / Yin, Rong; Rice, Shawn J.; Wang, Jinwei; Gao, Lin; Tsai, Joseph; Anvari, Radean T.; Zhou, Fang; Liu, Xin; Wang, Gang; Tang, Yuxin; Jr, Martin C.Mihm; Belani, Chandra P.; Chen, Dong Bao; Nelson, J. Stuart; Tan, Wenbin.

In: Histology and histopathology, Vol. 34, No. 5, 05.2019, p. 479-490.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels

AU - Yin, Rong

AU - Rice, Shawn J.

AU - Wang, Jinwei

AU - Gao, Lin

AU - Tsai, Joseph

AU - Anvari, Radean T.

AU - Zhou, Fang

AU - Liu, Xin

AU - Wang, Gang

AU - Tang, Yuxin

AU - Jr, Martin C.Mihm

AU - Belani, Chandra P.

AU - Chen, Dong Bao

AU - Nelson, J. Stuart

AU - Tan, Wenbin

PY - 2019/5

Y1 - 2019/5

N2 - Introduction. Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS. Methods. Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM). Results. 107 out of 299 identified proteins showed differential expressions in PWS lesions as compared to normal skin, mainly involving the functions of biosynthesis, membrane trafficking, cytoskeleton and cell adhesion/migration. The confirmative studies showed that expressions of membrane trafficking/ exocytosis related proteins such as VAT1, IQGAP1, HSC70, clathrin, perlecan, spectrin α1 and GDIR1 were significantly increased in PWS blood vessels as compared to normal ones. Furthermore, TEM studies showed there is a significant upregulation of extracellular vesicle exocytosis from PWS blood vessels as compared to control. Conclusions. The biological process of membrane trafficking and exocytosis is enhanced in PWS blood vessels. Our results imply that the extracellular vesicles released by lesional endothelial cells may act as potential intercellular signaling mediators to contribute to the pathogenesis of PWS.

AB - Introduction. Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS. Methods. Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM). Results. 107 out of 299 identified proteins showed differential expressions in PWS lesions as compared to normal skin, mainly involving the functions of biosynthesis, membrane trafficking, cytoskeleton and cell adhesion/migration. The confirmative studies showed that expressions of membrane trafficking/ exocytosis related proteins such as VAT1, IQGAP1, HSC70, clathrin, perlecan, spectrin α1 and GDIR1 were significantly increased in PWS blood vessels as compared to normal ones. Furthermore, TEM studies showed there is a significant upregulation of extracellular vesicle exocytosis from PWS blood vessels as compared to control. Conclusions. The biological process of membrane trafficking and exocytosis is enhanced in PWS blood vessels. Our results imply that the extracellular vesicles released by lesional endothelial cells may act as potential intercellular signaling mediators to contribute to the pathogenesis of PWS.

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