Membrane type matrix metalloproteinases (MMPs) show differential expression in non-small cell lung cancer (NSCLC) compared to normal lung: Correlation of MMP-14 mRNA expression and proteolytic activity

J. M. Atkinson, C. J. Pennington, S. W. Martin, V. A. Anikin, A. J. Mearns, P. M. Loadman, D. R. Edwards, J. H. Gill

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Abstract

Improved understanding of the involvement of matrix metalloproteinases (MMPs), including membrane-type MMPs (MT-MMPs), in human tumours has potential diagnostic, prognostic and therapeutic implications. We assessed the relationship between MT-MMP expression and clinicopathological parameters in human non-small cell lung cancer (NSCLC) and histologically normal lung tissue by quantitative Real Time PCR (qRT-PCR). All MT-MMPs (MMPs 14-17, 24 and 25) were detected by qRT-PCR with significantly higher MMP-14, -15 and -17 expression observed in tumour relative to normal lung specimens. MMP-16 was undetectable in normal lung but expressed in 8% tumours. MMP-15 demonstrated significant overexpression in adenocarcinomas relative to squamous cell carcinomas and normal lung tissue. MMP-14 mRNA expression strongly correlated to MMP-14 proteolytic activity in preclinical tumour models, indicating that qRT-PCR may predict MMP-14 activity levels in NSCLC. These data suggest that MMP-14, -15 and -17 may be good markers of disease, or therapeutic targets for treatment of human NSCLC.

Original languageEnglish (US)
Pages (from-to)1764-1771
Number of pages8
JournalEuropean Journal of Cancer
Volume43
Issue number11
DOIs
StatePublished - Jul 1 2007

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Membrane-Associated Matrix Metalloproteinases
Matrix Metalloproteinase 14
Non-Small Cell Lung Carcinoma
Matrix Metalloproteinase 15
Lung
Messenger RNA
Matrix Metalloproteinases
Real-Time Polymerase Chain Reaction
Matrix Metalloproteinase 17
Matrix Metalloproteinase 16
Neoplasms
Squamous Cell Carcinoma
Adenocarcinoma
Membranes
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Atkinson, J. M. ; Pennington, C. J. ; Martin, S. W. ; Anikin, V. A. ; Mearns, A. J. ; Loadman, P. M. ; Edwards, D. R. ; Gill, J. H. / Membrane type matrix metalloproteinases (MMPs) show differential expression in non-small cell lung cancer (NSCLC) compared to normal lung : Correlation of MMP-14 mRNA expression and proteolytic activity. In: European Journal of Cancer. 2007 ; Vol. 43, No. 11. pp. 1764-1771.
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abstract = "Improved understanding of the involvement of matrix metalloproteinases (MMPs), including membrane-type MMPs (MT-MMPs), in human tumours has potential diagnostic, prognostic and therapeutic implications. We assessed the relationship between MT-MMP expression and clinicopathological parameters in human non-small cell lung cancer (NSCLC) and histologically normal lung tissue by quantitative Real Time PCR (qRT-PCR). All MT-MMPs (MMPs 14-17, 24 and 25) were detected by qRT-PCR with significantly higher MMP-14, -15 and -17 expression observed in tumour relative to normal lung specimens. MMP-16 was undetectable in normal lung but expressed in 8{\%} tumours. MMP-15 demonstrated significant overexpression in adenocarcinomas relative to squamous cell carcinomas and normal lung tissue. MMP-14 mRNA expression strongly correlated to MMP-14 proteolytic activity in preclinical tumour models, indicating that qRT-PCR may predict MMP-14 activity levels in NSCLC. These data suggest that MMP-14, -15 and -17 may be good markers of disease, or therapeutic targets for treatment of human NSCLC.",
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Membrane type matrix metalloproteinases (MMPs) show differential expression in non-small cell lung cancer (NSCLC) compared to normal lung : Correlation of MMP-14 mRNA expression and proteolytic activity. / Atkinson, J. M.; Pennington, C. J.; Martin, S. W.; Anikin, V. A.; Mearns, A. J.; Loadman, P. M.; Edwards, D. R.; Gill, J. H.

In: European Journal of Cancer, Vol. 43, No. 11, 01.07.2007, p. 1764-1771.

Research output: Contribution to journalArticle

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AU - Martin, S. W.

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AU - Gill, J. H.

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