Mercury(II) bioaccumulation and antioxidant physiology in four aquatic insects

X. I.E. Lingtian, Jennifer L. Flippin, Nigel Deighton, David H. Funk, David A. Dickey, David B. Buchwalter

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We examined Hg(II) bioaccumulation and compartmentalization patterns in conjunction with antioxidant responses in four aquatic insect species: two caddisflies (Chimarra sp. and Hydropsyche betteni) and two mayflies (Maccaffertium modestum and Isonychia sp). Total antioxidant capabilities differed among unexposed larvae, with both caddisfly species exhibiting elevated antioxidant activities relative to the mayflies. We were able to account for these differences by examining the constitutive activities of catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and superoxide dismutase (SOD), in the four species. We also examined levels of reduced and oxidized glutathione and cysteine in the insects. Glutathione peroxidase and SOD were the most responsive to Hg exposure, with GPx catalytic activity increasing between 50 and 310%. Superoxide dismutase activity decreased between 35 and 50%. This SOD suppression was shown to be dose-dependent in both caddisflies, but the strength of this suppression did not appear to be related to rates of uptake. Surprisingly, little Hg (<10%) was found in the heat- stable cytosolic protein subcellular compartment in each of the four species, suggesting that Hg was not well detoxified. By combining bioaccumulation studies with other physiological measures, we can begin to better understand the consequences of trace metal pollutants in nature.

Original languageEnglish (US)
Pages (from-to)934-940
Number of pages7
JournalEnvironmental Science and Technology
Volume43
Issue number3
DOIs
StatePublished - Feb 1 2009

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Bioaccumulation
Physiology
Mercury
antioxidant
bioaccumulation
caddisfly
Superoxide Dismutase
physiology
Glutathione Peroxidase
Antioxidants
insect
mayfly
compartmentalization
Glutathione Disulfide
Glutathione Transferase
Catalase
trace metal
Glutathione
Cysteine
Catalyst activity

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Lingtian, X. I. E., Flippin, J. L., Deighton, N., Funk, D. H., Dickey, D. A., & Buchwalter, D. B. (2009). Mercury(II) bioaccumulation and antioxidant physiology in four aquatic insects. Environmental Science and Technology, 43(3), 934-940. https://doi.org/10.1021/es802323r
Lingtian, X. I.E. ; Flippin, Jennifer L. ; Deighton, Nigel ; Funk, David H. ; Dickey, David A. ; Buchwalter, David B. / Mercury(II) bioaccumulation and antioxidant physiology in four aquatic insects. In: Environmental Science and Technology. 2009 ; Vol. 43, No. 3. pp. 934-940.
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Lingtian, XIE, Flippin, JL, Deighton, N, Funk, DH, Dickey, DA & Buchwalter, DB 2009, 'Mercury(II) bioaccumulation and antioxidant physiology in four aquatic insects', Environmental Science and Technology, vol. 43, no. 3, pp. 934-940. https://doi.org/10.1021/es802323r

Mercury(II) bioaccumulation and antioxidant physiology in four aquatic insects. / Lingtian, X. I.E.; Flippin, Jennifer L.; Deighton, Nigel; Funk, David H.; Dickey, David A.; Buchwalter, David B.

In: Environmental Science and Technology, Vol. 43, No. 3, 01.02.2009, p. 934-940.

Research output: Contribution to journalArticle

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