Metabolism in vitro of dihydrotestosterone, 5α-androstane 3α, 17β-diol and its 3β-epimer, three metabolites of testosterone, by three of its target tissues, the anterior pituitary, the medial basal hypothalamus and the seminiferous tubules

Wei Liu Kao Lidia Wei Liu Kao, Anibal Perez Lloret, Judith Weisz

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Abstract

To examine the metabolism of three 5α-reduced derivatives of testosterone (T) by seminiferous tubules (ST), anterior pituitary (AP) and medial basal hypothalamus (MBH) of adult rats, these tissues were incubated with either tritiated dihydrotestosterone (DHT), α-androstan-3α, 17β diol (3α-adiol) or its 3β-epimer (3β-adiol). The products and residual precursors were quantified using 14C labeled tracers for recovery. Both DHT and 3β-adiol were extensively metabolized. The % DHT metabolized ranged from 60% (MBH) to 93% (ST) with 3α-adiol forming the major product (ST = 69%, AP = 54%, MBH = 38%). The % 3β-adiol metabolized ranged from 68% (ST) to 90% (AP). The metabolism of 3β-adiol by ST differed from that by the other tissues; reversibility of 3β-oxidoreduction, with substantial accumulation of DHT (13%) and 3α-adiol (36%) occurred only with ST. With AP and MBH these metabolites were identifiable only with increased substrate concentrations. In all incubations with AP and MBH with 3β-adiol the bulk of the radioactivity was associated with some polar metabolites (AP = 76%, MBH = 62%). An inverse relationship between polar metabolite formation and 3/gb-oxidation was suggested by the much smaller amounts of such metabolites being formed by ST. In all tissues the bulk of 3α-adiol remained unmetabolized. The % conversion of 3a-adiol to DHT was relatively high for AP and MBH (av = 27%) but low for ST (av = 7%).

Original languageEnglish (US)
Pages (from-to)1109-1115
Number of pages7
JournalJournal of Steroid Biochemistry
Volume8
Issue number10
DOIs
StatePublished - Oct 1977

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

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