Metabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental Chemicals Induces Systemic Metabolic Dysfunction in Mice

Limin Zhang, Emmanuel Hatzakis, Robert G. Nichols, Ruixin Hao, Jared Correll, Philip B. Smith, Christopher R. Chiaro, Gary H. Perdew, Andrew David Patterson

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Environmental exposure to dioxins and dioxin-like compounds poses a significant health risk for human health. Developing a better understanding of the mechanisms of toxicity through activation of the aryl hydrocarbon receptor (AHR) is likely to improve the reliability of risk assessment. In this study, the AHR-dependent metabolic response of mice exposed to 2,3,7,8-tetrachlorodibenzofuran (TCDF) was assessed using global 1H nuclear magnetic resonance (NMR)-based metabolomics and targeted metabolite profiling of extracts obtained from serum and liver. 1H NMR analyses revealed that TCDF exposure suppressed gluconeogenesis and glycogenolysis, stimulated lipogenesis, and triggered inflammatory gene expression in an Ahr-dependent manner. Targeted analyses using gas chromatography coupled with mass spectrometry showed TCDF treatment altered the ratio of unsaturated/saturated fatty acids. Consistent with this observation, an increase in hepatic expression of stearoyl coenzyme A desaturase 1 was observed. In addition, TCDF exposure resulted in inhibition of de novo fatty acid biosynthesis manifested by down-regulation of acetyl-CoA, malonyl-CoA, and palmitoyl-CoA metabolites and related mRNA levels. In contrast, no significant changes in the levels of glucose and lipid were observed in serum and liver obtained from Ahr-null mice following TCDF treatment, thus strongly supporting the important role of the AHR in mediating the metabolic effects seen following TCDF exposure. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)8067-8077
Number of pages11
JournalEnvironmental Science and Technology
Volume49
Issue number13
DOIs
StatePublished - Jul 7 2015

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Aryl Hydrocarbon Receptors
Dioxins
Chemical activation
hydrocarbon
Metabolites
Liver
Fatty Acids
Nuclear magnetic resonance
dioxin
Palmitoyl Coenzyme A
Malonyl Coenzyme A
Unsaturated fatty acids
nuclear magnetic resonance
serum
metabolite
Acetyl Coenzyme A
fatty acid
Health risks
Biosynthesis
Gene expression

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Zhang, Limin ; Hatzakis, Emmanuel ; Nichols, Robert G. ; Hao, Ruixin ; Correll, Jared ; Smith, Philip B. ; Chiaro, Christopher R. ; Perdew, Gary H. ; Patterson, Andrew David. / Metabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental Chemicals Induces Systemic Metabolic Dysfunction in Mice. In: Environmental Science and Technology. 2015 ; Vol. 49, No. 13. pp. 8067-8077.
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Metabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental Chemicals Induces Systemic Metabolic Dysfunction in Mice. / Zhang, Limin; Hatzakis, Emmanuel; Nichols, Robert G.; Hao, Ruixin; Correll, Jared; Smith, Philip B.; Chiaro, Christopher R.; Perdew, Gary H.; Patterson, Andrew David.

In: Environmental Science and Technology, Vol. 49, No. 13, 07.07.2015, p. 8067-8077.

Research output: Contribution to journalArticle

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AU - Zhang, Limin

AU - Hatzakis, Emmanuel

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