Metabolon formation by chemotaxis

Xi Zhao, Ayusman Sen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Enzymes that participate in reaction cascades have been shown to assemble into metabolons, in the presence of the first enzyme's substrate. However, the mechanism of metabolon formation is still an open question. We applied microfluidic and fluorescent spectroscopy techniques to study the coordinated movement of the first four enzymes of the glycolysis cycle: hexokinase, phosphoglucose isomerase, phosphofructokinase, and aldolase and showed that each enzyme independently follows its own specific substrate gradient, which is produced by the preceding enzymatic reaction. The extent of enzyme migration is proportional to the time the enzyme is exposed to the substrate gradient. Significantly, the chemotactic migration of enzymes is fairly rapid even under conditions that mimic cytosolic crowding. The observed rate was very similar to the reported rate of enzyme diffusion in living cells. Thus, chemotaxis may be a basis for the organization of metabolic networks in the cytosol of the cell.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages45-62
Number of pages18
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Enzymology
Volume617
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

Chemotaxis
Enzymes
Substrates
Glucose-6-Phosphate Isomerase
Phosphofructokinases
Fructose-Bisphosphate Aldolase
Crowding
Hexokinase
Microfluidics
Glycolysis
Metabolic Networks and Pathways
Cytosol
Spectrum Analysis
Cells
Spectroscopy

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Zhao, X., & Sen, A. (2019). Metabolon formation by chemotaxis. In Methods in Enzymology (pp. 45-62). (Methods in Enzymology; Vol. 617). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2018.12.003
Zhao, Xi ; Sen, Ayusman. / Metabolon formation by chemotaxis. Methods in Enzymology. Academic Press Inc., 2019. pp. 45-62 (Methods in Enzymology).
@inbook{6d7cd9b6b158451fa13fac702c42f9aa,
title = "Metabolon formation by chemotaxis",
abstract = "Enzymes that participate in reaction cascades have been shown to assemble into metabolons, in the presence of the first enzyme's substrate. However, the mechanism of metabolon formation is still an open question. We applied microfluidic and fluorescent spectroscopy techniques to study the coordinated movement of the first four enzymes of the glycolysis cycle: hexokinase, phosphoglucose isomerase, phosphofructokinase, and aldolase and showed that each enzyme independently follows its own specific substrate gradient, which is produced by the preceding enzymatic reaction. The extent of enzyme migration is proportional to the time the enzyme is exposed to the substrate gradient. Significantly, the chemotactic migration of enzymes is fairly rapid even under conditions that mimic cytosolic crowding. The observed rate was very similar to the reported rate of enzyme diffusion in living cells. Thus, chemotaxis may be a basis for the organization of metabolic networks in the cytosol of the cell.",
author = "Xi Zhao and Ayusman Sen",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/bs.mie.2018.12.003",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "45--62",
booktitle = "Methods in Enzymology",
address = "United States",

}

Zhao, X & Sen, A 2019, Metabolon formation by chemotaxis. in Methods in Enzymology. Methods in Enzymology, vol. 617, Academic Press Inc., pp. 45-62. https://doi.org/10.1016/bs.mie.2018.12.003

Metabolon formation by chemotaxis. / Zhao, Xi; Sen, Ayusman.

Methods in Enzymology. Academic Press Inc., 2019. p. 45-62 (Methods in Enzymology; Vol. 617).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Metabolon formation by chemotaxis

AU - Zhao, Xi

AU - Sen, Ayusman

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Enzymes that participate in reaction cascades have been shown to assemble into metabolons, in the presence of the first enzyme's substrate. However, the mechanism of metabolon formation is still an open question. We applied microfluidic and fluorescent spectroscopy techniques to study the coordinated movement of the first four enzymes of the glycolysis cycle: hexokinase, phosphoglucose isomerase, phosphofructokinase, and aldolase and showed that each enzyme independently follows its own specific substrate gradient, which is produced by the preceding enzymatic reaction. The extent of enzyme migration is proportional to the time the enzyme is exposed to the substrate gradient. Significantly, the chemotactic migration of enzymes is fairly rapid even under conditions that mimic cytosolic crowding. The observed rate was very similar to the reported rate of enzyme diffusion in living cells. Thus, chemotaxis may be a basis for the organization of metabolic networks in the cytosol of the cell.

AB - Enzymes that participate in reaction cascades have been shown to assemble into metabolons, in the presence of the first enzyme's substrate. However, the mechanism of metabolon formation is still an open question. We applied microfluidic and fluorescent spectroscopy techniques to study the coordinated movement of the first four enzymes of the glycolysis cycle: hexokinase, phosphoglucose isomerase, phosphofructokinase, and aldolase and showed that each enzyme independently follows its own specific substrate gradient, which is produced by the preceding enzymatic reaction. The extent of enzyme migration is proportional to the time the enzyme is exposed to the substrate gradient. Significantly, the chemotactic migration of enzymes is fairly rapid even under conditions that mimic cytosolic crowding. The observed rate was very similar to the reported rate of enzyme diffusion in living cells. Thus, chemotaxis may be a basis for the organization of metabolic networks in the cytosol of the cell.

UR - http://www.scopus.com/inward/record.url?scp=85060920983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060920983&partnerID=8YFLogxK

U2 - 10.1016/bs.mie.2018.12.003

DO - 10.1016/bs.mie.2018.12.003

M3 - Chapter

C2 - 30784413

AN - SCOPUS:85060920983

T3 - Methods in Enzymology

SP - 45

EP - 62

BT - Methods in Enzymology

PB - Academic Press Inc.

ER -

Zhao X, Sen A. Metabolon formation by chemotaxis. In Methods in Enzymology. Academic Press Inc. 2019. p. 45-62. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2018.12.003