Metagenomic analysis of the fecal microbiomes from Escherichia coli O157:H7-shedding and non-shedding cows on a single dairy farm

Serajus Salaheen, Seon Woo Kim, Jeffrey S. Karns, Ernest Hovingh, Bradd J. Haley, Jo Ann S. Van Kessel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Escherichia coli O157:H7 (O157:H7) is a major zoonotic pathogen responsible for severe and sometimes fatal infections in humans. Cattle are the primary reservoir of O157:H7, although other animal species are also known carriers. Fecal shedding of this pathogen can potentially lead to contamination of pre-pasteurized milk, meat, water, produce, wildlife, and the environment. Understanding the relationships between O157:H7 carriage and the structure of the ruminant intestinal microbiome may inform development of mitigation strategies. To assess the compositional differences in the fecal microbiota of O157:H7-shedding and non-shedding cows, 14 fecal samples from lactating cows in the same dairy herd were analyzed using shotgun metagenomic sequencing. Results indicated that the microbial community, resistome, and virulome structures did not differ based on shedding status, but several unique taxa were identified in the fecal metagenomes in at-least 50% of the samples from the O157:H7-shedding cows. Further, differential relative abundance of 20 taxa belonging to the phyla Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Spirochaetes, Cyanobacteria, and Verrucomicrobia, were observed in the fecal samples between the two groups. The results of this study indicate that subtle differences in the fecal metagenomes of lactating dairy cows may be associated with E. coli O157:H7 shedding.

Original languageEnglish (US)
Pages (from-to)76-80
Number of pages5
JournalFood Control
Volume102
DOIs
StatePublished - Aug 2019

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Metagenome
Metagenomics
Escherichia coli O157
Microbiota
dairy farming
Verrucomicrobia
Bacteroidetes
cows
Proteobacteria
Spirochaetales
Actinobacteria
Zoonoses
Firearms
Ruminants
Cyanobacteria
Meat
Milk
Firmicutes
pasteurized milk
pathogens

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science

Cite this

Salaheen, Serajus ; Kim, Seon Woo ; Karns, Jeffrey S. ; Hovingh, Ernest ; Haley, Bradd J. ; Van Kessel, Jo Ann S. / Metagenomic analysis of the fecal microbiomes from Escherichia coli O157:H7-shedding and non-shedding cows on a single dairy farm. In: Food Control. 2019 ; Vol. 102. pp. 76-80.
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abstract = "Escherichia coli O157:H7 (O157:H7) is a major zoonotic pathogen responsible for severe and sometimes fatal infections in humans. Cattle are the primary reservoir of O157:H7, although other animal species are also known carriers. Fecal shedding of this pathogen can potentially lead to contamination of pre-pasteurized milk, meat, water, produce, wildlife, and the environment. Understanding the relationships between O157:H7 carriage and the structure of the ruminant intestinal microbiome may inform development of mitigation strategies. To assess the compositional differences in the fecal microbiota of O157:H7-shedding and non-shedding cows, 14 fecal samples from lactating cows in the same dairy herd were analyzed using shotgun metagenomic sequencing. Results indicated that the microbial community, resistome, and virulome structures did not differ based on shedding status, but several unique taxa were identified in the fecal metagenomes in at-least 50{\%} of the samples from the O157:H7-shedding cows. Further, differential relative abundance of 20 taxa belonging to the phyla Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Spirochaetes, Cyanobacteria, and Verrucomicrobia, were observed in the fecal samples between the two groups. The results of this study indicate that subtle differences in the fecal metagenomes of lactating dairy cows may be associated with E. coli O157:H7 shedding.",
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Metagenomic analysis of the fecal microbiomes from Escherichia coli O157:H7-shedding and non-shedding cows on a single dairy farm. / Salaheen, Serajus; Kim, Seon Woo; Karns, Jeffrey S.; Hovingh, Ernest; Haley, Bradd J.; Van Kessel, Jo Ann S.

In: Food Control, Vol. 102, 08.2019, p. 76-80.

Research output: Contribution to journalArticle

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