Metagenomic Detection of Viruses in Aerosol Samples from Workers in Animal Slaughterhouses

Richard J. Hall, Mily Leblanc-Maridor, Jing Wang, Xiaoyun Ren, Nicole E. Moore, Collin R. Brooks, Matthew Peacey, Jeroen Douwes, David J. McLean

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Published studies have shown that workers in animal slaughterhouses are at a higher risk of lung cancers as compared to the general population. No specific causal agents have been identified, and exposures to several chemicals have been examined and found to be unrelated. Evidence suggests a biological aetiology as the risk is highest for workers who are exposed to live animals or to biological material containing animal faeces, urine or blood. To investigate possible biological exposures in animal slaughterhouses, we used a metagenomic approach to characterise the profile of organisms present within an aerosol sample. An assessment of aerosol exposures for individual workers was achieved by the collection of personal samples that represent the inhalable fraction of dust/bioaerosol in workplace air in both cattle and sheep slaughterhouses. Two sets of nine personal aerosol samples were pooled for the cattle processing and sheep processing areas respectively, with a total of 332,677,346 sequence reads and 250,144,492 sequence reads of 85 bp in length produced for each. Eukaryotic genome sequence was found in both sampling locations, and bovine, ovine and human sequences were common. Sequences from WU polyomavirus and human papillomavirus 120 were detected in the metagenomic dataset from the cattle processing area, and these sequences were confirmed as being present in the original personal aerosol samples. This study presents the first metagenomic description of personal aerosol exposure and this methodology could be applied to a variety of environments. Also, the detection of two candidate viruses warrants further investigation in the setting of occupational exposures in animal slaughterhouses.

Original languageEnglish (US)
Article numbere72226
JournalPloS one
Volume8
Issue number8
DOIs
StatePublished - Aug 14 2013

Fingerprint

Metagenomics
Abattoirs
aerosols
Aerosols
Viruses
slaughterhouses
Animals
viruses
Sheep
cattle
animals
sheep
sampling
Processing
Polyomavirus
bioaerosols
occupational exposure
Papillomaviridae
working conditions
Occupational Exposure

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Hall, R. J., Leblanc-Maridor, M., Wang, J., Ren, X., Moore, N. E., Brooks, C. R., ... McLean, D. J. (2013). Metagenomic Detection of Viruses in Aerosol Samples from Workers in Animal Slaughterhouses. PloS one, 8(8), [e72226]. https://doi.org/10.1371/journal.pone.0072226
Hall, Richard J. ; Leblanc-Maridor, Mily ; Wang, Jing ; Ren, Xiaoyun ; Moore, Nicole E. ; Brooks, Collin R. ; Peacey, Matthew ; Douwes, Jeroen ; McLean, David J. / Metagenomic Detection of Viruses in Aerosol Samples from Workers in Animal Slaughterhouses. In: PloS one. 2013 ; Vol. 8, No. 8.
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Hall, RJ, Leblanc-Maridor, M, Wang, J, Ren, X, Moore, NE, Brooks, CR, Peacey, M, Douwes, J & McLean, DJ 2013, 'Metagenomic Detection of Viruses in Aerosol Samples from Workers in Animal Slaughterhouses', PloS one, vol. 8, no. 8, e72226. https://doi.org/10.1371/journal.pone.0072226

Metagenomic Detection of Viruses in Aerosol Samples from Workers in Animal Slaughterhouses. / Hall, Richard J.; Leblanc-Maridor, Mily; Wang, Jing; Ren, Xiaoyun; Moore, Nicole E.; Brooks, Collin R.; Peacey, Matthew; Douwes, Jeroen; McLean, David J.

In: PloS one, Vol. 8, No. 8, e72226, 14.08.2013.

Research output: Contribution to journalArticle

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