Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosinederived dihydroxyphenylalanine radical

Elizabeth J. Blaesi, Gavin M. Palowitch, Kai Hu, Amelia J. Kim, Hannah R. Rose, Rahul Alapati, Marshall G. Lougee, Hee Jong Kim, Alexander T. Taguchi, Kong Ooi Tan, Tatiana Nikolaevna Laremore, Robert G. Griffin, Carsten Krebs, Megan L. Matthews, Alexey Silakov, Joseph M. Bollinger, Jr., Benjamin D. Allen, Amie Kathleen Boal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

All cells obtain 2′-deoxyribonucleotides for DNA synthesis through the activity of a ribonucleotide reductase (RNR). The class I RNRs found in humans and pathogenic bacteria differ in (i) use of Fe(II), Mn(II), or both for activation of the dinuclear-metallocofactor subunit, β; (ii) reaction of the reduced dimetal center with dioxygen or superoxide for this activation; (iii) requirement (or lack thereof) for a flavoprotein activase, NrdI, to provide the superoxide from O2; and (iv) use of either a stable tyrosyl radical or a high-valent dimetal cluster to initiate each turnover by oxidizing a cysteine residue in the α subunit to a radical (Cys•). The use of manganese by bacterial class I, subclass b-d RNRs, which contrasts with the exclusive use of iron by the eukaryotic Ia enzymes, appears to be a countermeasure of certain pathogens against iron deprivation imposed by their hosts. Here, we report a metal-free type of class I RNR (subclass e) from two human pathogens. The Cys• in its α subunit is generated by a stable, tyrosine-derived dihydroxyphenylalanine radical (DOPA•) in β. The three-electron oxidation producing DOPA• occurs in Escherichia coli only if the β is coexpressed with the NrdI activase encoded adjacently in the pathogen genome. The independence of this new RNR from transition metals, or the requirement for a single metal ion only transiently for activation, may afford the pathogens an even more potent countermeasure against transition metal-directed innate immunity.

Original languageEnglish (US)
Pages (from-to)10022-10027
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number40
DOIs
StatePublished - Oct 2 2018

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Ribonucleotide Reductases
Dihydroxyphenylalanine
Catalysis
Metals
Tissue Plasminogen Activator
Superoxides
Iron
Deoxyribonucleotides
Flavoproteins
Manganese
Innate Immunity
Cysteine
Tyrosine
Genome
Electrons
Ions
Oxygen
Escherichia coli
Bacteria
DNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

Blaesi, Elizabeth J. ; Palowitch, Gavin M. ; Hu, Kai ; Kim, Amelia J. ; Rose, Hannah R. ; Alapati, Rahul ; Lougee, Marshall G. ; Kim, Hee Jong ; Taguchi, Alexander T. ; Tan, Kong Ooi ; Laremore, Tatiana Nikolaevna ; Griffin, Robert G. ; Krebs, Carsten ; Matthews, Megan L. ; Silakov, Alexey ; Bollinger, Jr., Joseph M. ; Allen, Benjamin D. ; Boal, Amie Kathleen. / Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosinederived dihydroxyphenylalanine radical. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 40. pp. 10022-10027.
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abstract = "All cells obtain 2′-deoxyribonucleotides for DNA synthesis through the activity of a ribonucleotide reductase (RNR). The class I RNRs found in humans and pathogenic bacteria differ in (i) use of Fe(II), Mn(II), or both for activation of the dinuclear-metallocofactor subunit, β; (ii) reaction of the reduced dimetal center with dioxygen or superoxide for this activation; (iii) requirement (or lack thereof) for a flavoprotein activase, NrdI, to provide the superoxide from O2; and (iv) use of either a stable tyrosyl radical or a high-valent dimetal cluster to initiate each turnover by oxidizing a cysteine residue in the α subunit to a radical (Cys•). The use of manganese by bacterial class I, subclass b-d RNRs, which contrasts with the exclusive use of iron by the eukaryotic Ia enzymes, appears to be a countermeasure of certain pathogens against iron deprivation imposed by their hosts. Here, we report a metal-free type of class I RNR (subclass e) from two human pathogens. The Cys• in its α subunit is generated by a stable, tyrosine-derived dihydroxyphenylalanine radical (DOPA•) in β. The three-electron oxidation producing DOPA• occurs in Escherichia coli only if the β is coexpressed with the NrdI activase encoded adjacently in the pathogen genome. The independence of this new RNR from transition metals, or the requirement for a single metal ion only transiently for activation, may afford the pathogens an even more potent countermeasure against transition metal-directed innate immunity.",
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Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosinederived dihydroxyphenylalanine radical. / Blaesi, Elizabeth J.; Palowitch, Gavin M.; Hu, Kai; Kim, Amelia J.; Rose, Hannah R.; Alapati, Rahul; Lougee, Marshall G.; Kim, Hee Jong; Taguchi, Alexander T.; Tan, Kong Ooi; Laremore, Tatiana Nikolaevna; Griffin, Robert G.; Krebs, Carsten; Matthews, Megan L.; Silakov, Alexey; Bollinger, Jr., Joseph M.; Allen, Benjamin D.; Boal, Amie Kathleen.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 40, 02.10.2018, p. 10022-10027.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosinederived dihydroxyphenylalanine radical

AU - Blaesi, Elizabeth J.

AU - Palowitch, Gavin M.

AU - Hu, Kai

AU - Kim, Amelia J.

AU - Rose, Hannah R.

AU - Alapati, Rahul

AU - Lougee, Marshall G.

AU - Kim, Hee Jong

AU - Taguchi, Alexander T.

AU - Tan, Kong Ooi

AU - Laremore, Tatiana Nikolaevna

AU - Griffin, Robert G.

AU - Krebs, Carsten

AU - Matthews, Megan L.

AU - Silakov, Alexey

AU - Bollinger, Jr., Joseph M.

AU - Allen, Benjamin D.

AU - Boal, Amie Kathleen

PY - 2018/10/2

Y1 - 2018/10/2

N2 - All cells obtain 2′-deoxyribonucleotides for DNA synthesis through the activity of a ribonucleotide reductase (RNR). The class I RNRs found in humans and pathogenic bacteria differ in (i) use of Fe(II), Mn(II), or both for activation of the dinuclear-metallocofactor subunit, β; (ii) reaction of the reduced dimetal center with dioxygen or superoxide for this activation; (iii) requirement (or lack thereof) for a flavoprotein activase, NrdI, to provide the superoxide from O2; and (iv) use of either a stable tyrosyl radical or a high-valent dimetal cluster to initiate each turnover by oxidizing a cysteine residue in the α subunit to a radical (Cys•). The use of manganese by bacterial class I, subclass b-d RNRs, which contrasts with the exclusive use of iron by the eukaryotic Ia enzymes, appears to be a countermeasure of certain pathogens against iron deprivation imposed by their hosts. Here, we report a metal-free type of class I RNR (subclass e) from two human pathogens. The Cys• in its α subunit is generated by a stable, tyrosine-derived dihydroxyphenylalanine radical (DOPA•) in β. The three-electron oxidation producing DOPA• occurs in Escherichia coli only if the β is coexpressed with the NrdI activase encoded adjacently in the pathogen genome. The independence of this new RNR from transition metals, or the requirement for a single metal ion only transiently for activation, may afford the pathogens an even more potent countermeasure against transition metal-directed innate immunity.

AB - All cells obtain 2′-deoxyribonucleotides for DNA synthesis through the activity of a ribonucleotide reductase (RNR). The class I RNRs found in humans and pathogenic bacteria differ in (i) use of Fe(II), Mn(II), or both for activation of the dinuclear-metallocofactor subunit, β; (ii) reaction of the reduced dimetal center with dioxygen or superoxide for this activation; (iii) requirement (or lack thereof) for a flavoprotein activase, NrdI, to provide the superoxide from O2; and (iv) use of either a stable tyrosyl radical or a high-valent dimetal cluster to initiate each turnover by oxidizing a cysteine residue in the α subunit to a radical (Cys•). The use of manganese by bacterial class I, subclass b-d RNRs, which contrasts with the exclusive use of iron by the eukaryotic Ia enzymes, appears to be a countermeasure of certain pathogens against iron deprivation imposed by their hosts. Here, we report a metal-free type of class I RNR (subclass e) from two human pathogens. The Cys• in its α subunit is generated by a stable, tyrosine-derived dihydroxyphenylalanine radical (DOPA•) in β. The three-electron oxidation producing DOPA• occurs in Escherichia coli only if the β is coexpressed with the NrdI activase encoded adjacently in the pathogen genome. The independence of this new RNR from transition metals, or the requirement for a single metal ion only transiently for activation, may afford the pathogens an even more potent countermeasure against transition metal-directed innate immunity.

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DO - 10.1073/pnas.1811993115

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JF - Proceedings of the National Academy of Sciences of the United States of America

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