Metal Ion Catalysis of Phosphoryl Transfer from Phosphoenolpyruvate

S. J. Benkovic, K. J. Schray

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Abstract

The catalytic effect of mercury(II) and a number of other metal ions on the hydrolysis of phosphoenolpyruvate and ethyl phosphoenolpyruvate has been investigated. The mechanisms of catalysis and their effect on the phosphorylating properties of these substrate have been determined via kinetic and product studies. The unusual properties of the mercury(II)-phosphoenolpyruvate reaction which proceeds via mercury(II) addition to the enol double bond and can lead to either phosphorylation of solvent or Pi release are discussed. The implications of these studies for the mechanism of the pyruvate kinase reaction are considered.

Original languageEnglish (US)
Pages (from-to)4097-4102
Number of pages6
JournalBiochemistry
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 1 1968

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All Science Journal Classification (ASJC) codes

  • Biochemistry

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