Metal reduction at low pH by a Desulfosporosinusspecies: Implications for the biological treatment of acidic mine drainage

John M. Senko, Gengxin Zhang, Jeffrey T. McDonough, Maryann Victoria Bruns, William D. Burgos

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We isolated an acid-tolerant sulfate-reducing bacterium, GBSRB4.2, from coal mine-derived acidic mine drainage (AMD)-derived sediments. Sequence analysis of partial 16S rRNA gene of GBSRB4.2 revealed that it was affiliated with the genus Desulfosporosinus. GBSRB4.2 reduced sulfate, Fe(III) (hydr)oxide, Mn(IV) oxide, and U(VI) in acidic solutions (pH 4.2). Sulfate, Fe(III), and Mn(IV) but not U(VI) bioreduction led to an increase in the pH of acidic solutions and concurrent hydrolysis and precipitation of dissolved Al3+. Reduction of Fe(III), Mn(IV), and U(VI) in sulfate-free solutions revealed that these metals are enzymatically reduced by GBSRB4.2. GBSRB4.2 reduced U(VI) in groundwater from a radionuclide-contaminated aquifer more rapidly at pH 4.4 than at pH 7.1, possibly due to the formation of poorly bioreducible Ca-U(VI)-CO3 complexes in the pH 7.1 groundwater.

Original languageEnglish (US)
Pages (from-to)71-82
Number of pages12
JournalGeomicrobiology Journal
Volume26
Issue number2
DOIs
StatePublished - Mar 6 2009

Fingerprint

mine drainage
Sulfates
Drainage
Metals
sulfate
Groundwater
metal
oxide
Oxides
groundwater
sulfate-reducing bacterium
coal mine
radionuclide
hydrolysis
Therapeutics
Coal mines
Aquifers
Radioisotopes
aquifer
Coal

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Environmental Chemistry
  • Environmental Science(all)
  • Earth and Planetary Sciences (miscellaneous)

Cite this

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abstract = "We isolated an acid-tolerant sulfate-reducing bacterium, GBSRB4.2, from coal mine-derived acidic mine drainage (AMD)-derived sediments. Sequence analysis of partial 16S rRNA gene of GBSRB4.2 revealed that it was affiliated with the genus Desulfosporosinus. GBSRB4.2 reduced sulfate, Fe(III) (hydr)oxide, Mn(IV) oxide, and U(VI) in acidic solutions (pH 4.2). Sulfate, Fe(III), and Mn(IV) but not U(VI) bioreduction led to an increase in the pH of acidic solutions and concurrent hydrolysis and precipitation of dissolved Al3+. Reduction of Fe(III), Mn(IV), and U(VI) in sulfate-free solutions revealed that these metals are enzymatically reduced by GBSRB4.2. GBSRB4.2 reduced U(VI) in groundwater from a radionuclide-contaminated aquifer more rapidly at pH 4.4 than at pH 7.1, possibly due to the formation of poorly bioreducible Ca-U(VI)-CO3 complexes in the pH 7.1 groundwater.",
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Metal reduction at low pH by a Desulfosporosinusspecies : Implications for the biological treatment of acidic mine drainage. / Senko, John M.; Zhang, Gengxin; McDonough, Jeffrey T.; Bruns, Maryann Victoria; Burgos, William D.

In: Geomicrobiology Journal, Vol. 26, No. 2, 06.03.2009, p. 71-82.

Research output: Contribution to journalArticle

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