Methane-consuming archaea revealed by directly coupled isotopic and phylogenetic analysis

V. J. Orphan, C. H. House, K. U. Hinrichs, K. D. McKeegan, E. F. DeLong

Research output: Contribution to journalArticlepeer-review

735 Scopus citations

Abstract

Microorganisms living in anoxic marine sediments consume more than 80% of the methane produced in the world's oceans. In addition to single-species aggregates, consortia of metabolically interdependent bacteria and archaea are found in methane-rich sediments. A combination of fluorescence in situ hybridization and secondary ion mass spectrometry shows that cells belonging to one specific archaeal group associated with the Methanosarcinales were all highly depleted in 13C (to values of -96‰). This depletion indicates assimilation of isotopically light methane into specific archaeal cells. Additional microbial species apparently use other carbon sources, as indicated by significantly higher 13C/12C ratios in their cell carbon. Our results demonstrate the feasibility of simultaneous determination of the identity and the metabolic activity of naturally occurring microorganisms.

Original languageEnglish (US)
Pages (from-to)484-487
Number of pages4
JournalScience
Volume293
Issue number5529
DOIs
StatePublished - Jul 20 2001

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Methane-consuming archaea revealed by directly coupled isotopic and phylogenetic analysis'. Together they form a unique fingerprint.

Cite this