Methodology for the Identification and Purification of Taxol and Cephalomannine From Taxus Callus Cultures

Enaksha R.M. Wickremesenhe, Richard N. Arteca

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this study we have developed a new protocol for the extraction and purification of taxol and cephalomannine from plant tissue culture samples. This protocol allows large numbers of samples to be processed with a minimum use of solvents and can be completed in a relatively short period of time, compared to existing published protocols. The procedure involves a methanol extraction followed by the use of C18 Sep-pak cartridges, before analysis by isocratic high performance liquid chromatography (HPLC) using a reverse-phase analytical phenyl column. Our purification protocol consisted of a 65% methanol: chloroform partitioning step followed by C18 bonded silica column chromatography and finally, C18 preparatory HPLC.

Original languageEnglish (US)
Pages (from-to)3263-3274
Number of pages12
JournalJournal of Liquid Chromatography
Volume16
Issue number15
DOIs
StatePublished - Jan 1 1993

Fingerprint

Taxus
Bony Callus
High performance liquid chromatography
Paclitaxel
Purification
Methanol
High Pressure Liquid Chromatography
Tissue culture
Column chromatography
Distillation columns
Chloroform
Silicon Dioxide
Chromatography
cephalomannine
octadecyl silane-bonded silica

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

Cite this

Wickremesenhe, Enaksha R.M. ; Arteca, Richard N. / Methodology for the Identification and Purification of Taxol and Cephalomannine From Taxus Callus Cultures. In: Journal of Liquid Chromatography. 1993 ; Vol. 16, No. 15. pp. 3263-3274.
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Methodology for the Identification and Purification of Taxol and Cephalomannine From Taxus Callus Cultures. / Wickremesenhe, Enaksha R.M.; Arteca, Richard N.

In: Journal of Liquid Chromatography, Vol. 16, No. 15, 01.01.1993, p. 3263-3274.

Research output: Contribution to journalArticle

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