Methylation of estradiol-17β by a partially purified preparation of bovine pineal hydroxy-indole-o-methyltransferase

Judith Weisz, Laura V. O’Brien, Tom Lloyd

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A partially purified preparation of hydroxy-indole-O-methyltransferase (HIOMT) from bovine pineal was shown to O-methylate estradiol-17β (E2). The HIOMT preparation was incubated with E2 and [3H]-S-adenosylmethionine (SAM). The [3H]-3 methyl-ether of estradiol [MeO-E2] produced was identified by thin layer chromatography (TLC) and crystallization to constant 3H/14C ratio in the presence of [14C]-Me0-E2 and unlabeled MeO-E2 standards. The kinetics of the enzyme reaction for melatonin formation from N-acetylserotonin (NAS) and for MeO-E2 were compared.

Original languageEnglish (US)
Pages (from-to)330-333
Number of pages4
JournalEndocrinology
Volume102
Issue number1
DOIs
StatePublished - Jan 1978

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Methylation
Estradiol
Methyl Ethers
S-Adenosylmethionine
Melatonin
Thin Layer Chromatography
Crystallization
Enzymes
indole methyltransferase
N-acetylserotonin

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

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abstract = "A partially purified preparation of hydroxy-indole-O-methyltransferase (HIOMT) from bovine pineal was shown to O-methylate estradiol-17β (E2). The HIOMT preparation was incubated with E2 and [3H]-S-adenosylmethionine (SAM). The [3H]-3 methyl-ether of estradiol [MeO-E2] produced was identified by thin layer chromatography (TLC) and crystallization to constant 3H/14C ratio in the presence of [14C]-Me0-E2 and unlabeled MeO-E2 standards. The kinetics of the enzyme reaction for melatonin formation from N-acetylserotonin (NAS) and for MeO-E2 were compared.",
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Methylation of estradiol-17β by a partially purified preparation of bovine pineal hydroxy-indole-o-methyltransferase. / Weisz, Judith; O’Brien, Laura V.; Lloyd, Tom.

In: Endocrinology, Vol. 102, No. 1, 01.1978, p. 330-333.

Research output: Contribution to journalArticle

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T1 - Methylation of estradiol-17β by a partially purified preparation of bovine pineal hydroxy-indole-o-methyltransferase

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AB - A partially purified preparation of hydroxy-indole-O-methyltransferase (HIOMT) from bovine pineal was shown to O-methylate estradiol-17β (E2). The HIOMT preparation was incubated with E2 and [3H]-S-adenosylmethionine (SAM). The [3H]-3 methyl-ether of estradiol [MeO-E2] produced was identified by thin layer chromatography (TLC) and crystallization to constant 3H/14C ratio in the presence of [14C]-Me0-E2 and unlabeled MeO-E2 standards. The kinetics of the enzyme reaction for melatonin formation from N-acetylserotonin (NAS) and for MeO-E2 were compared.

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