Methylene blue counteracts cyanide cardiotoxicity: Cellular mechanisms

Joseph Y. Cheung, Jufang Wang, Xue Qian Zhang, Jianliang Song, Dhanendra Tomar, Muniswamy Madesh, Annick Judenherc-Haouzi, Philippe Haouzi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In adult left ventricular mouse myocytes, exposure to sodium cyanide (NaCN) in the presence of glucose dose-dependently reduced contraction amplitude, with ∼80% of maximal inhibitory effect attained at 100 μM. NaCN (100 μM) exposure for 10 min significantly decreased contraction and intracellular Ca2+ concentration ([Ca2+]i) transient amplitudes, systolic but not diastolic [Ca2+]i, and maximal L-type Ca2+ current (ICa) amplitude, indicating acute alteration of [Ca2+]i homeostasis largely accounted for the observed excitation-contraction abnormalities. In addition, NaCN depolarized resting membrane potential (Em), reduced action potential (AP) amplitude, prolonged AP duration at 50% (APD50) and 90% repolarization (APD90), and suppressed depolarization-activated K+ currents but had no effect on Na+-Ca2+ exchange current (INaCa). NaCN did not affect cellular adenosine triphosphate levels but depolarized mitochondrial membrane potential (Δψm) and increased superoxide (O2-) levels. Methylene blue (MB; 20 μg/ml) added 3 min after NaCN restored contraction and [Ca2+]i transient amplitudes, systolic [Ca2+]i, Em, AP amplitude, APD50, APD90, ICa, depolarization-activated K+ currents, Δψm, and O2- levels toward normal. We conclude that MB reversed NaCN-induced cardiotoxicity by preserving intracellular Ca2+ homeostasis and excitation-contraction coupling (ICa), minimizing risks of arrhythmias (Em, AP configuration, and depolarization-activated K+ currents), and reducing O2- levels. NEW & NOTEWORTHY Cyanide poisoning due to industrial exposure, smoke inhalation, and bioterrorism manifests as cardiogenic shock and requires rapidly effective antidote. In the early stage of cyanide exposure, adenosine triphosphate levels are normal but myocyte contractility is reduced, largely due to alterations in Ca2+ homeostasis because of changes in oxidation-reduction environment of ion channels. Methylene blue, a drug approved by the U.S. Food and Drug Administration, ameliorates cyanide toxicity by normalizing oxidation-reduction state and Ca2+ channel function.

Original languageEnglish (US)
Pages (from-to)1164-1176
Number of pages13
JournalJournal of applied physiology
Volume124
Issue number5
DOIs
StatePublished - May 2018

Fingerprint

Methylene Blue
Cyanides
Action Potentials
Homeostasis
Muscle Cells
Oxidation-Reduction
Adenosine Triphosphate
Sodium Cyanide
Bioterrorism
Inhalation Exposure
Excitation Contraction Coupling
Antidotes
Cardiogenic Shock
Mitochondrial Membrane Potential
United States Food and Drug Administration
Ion Channels
Smoke
Superoxides
Membrane Potentials
Poisoning

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Cheung, Joseph Y. ; Wang, Jufang ; Zhang, Xue Qian ; Song, Jianliang ; Tomar, Dhanendra ; Madesh, Muniswamy ; Judenherc-Haouzi, Annick ; Haouzi, Philippe. / Methylene blue counteracts cyanide cardiotoxicity : Cellular mechanisms. In: Journal of applied physiology. 2018 ; Vol. 124, No. 5. pp. 1164-1176.
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abstract = "In adult left ventricular mouse myocytes, exposure to sodium cyanide (NaCN) in the presence of glucose dose-dependently reduced contraction amplitude, with ∼80{\%} of maximal inhibitory effect attained at 100 μM. NaCN (100 μM) exposure for 10 min significantly decreased contraction and intracellular Ca2+ concentration ([Ca2+]i) transient amplitudes, systolic but not diastolic [Ca2+]i, and maximal L-type Ca2+ current (ICa) amplitude, indicating acute alteration of [Ca2+]i homeostasis largely accounted for the observed excitation-contraction abnormalities. In addition, NaCN depolarized resting membrane potential (Em), reduced action potential (AP) amplitude, prolonged AP duration at 50{\%} (APD50) and 90{\%} repolarization (APD90), and suppressed depolarization-activated K+ currents but had no effect on Na+-Ca2+ exchange current (INaCa). NaCN did not affect cellular adenosine triphosphate levels but depolarized mitochondrial membrane potential (Δψm) and increased superoxide (O2-) levels. Methylene blue (MB; 20 μg/ml) added 3 min after NaCN restored contraction and [Ca2+]i transient amplitudes, systolic [Ca2+]i, Em, AP amplitude, APD50, APD90, ICa, depolarization-activated K+ currents, Δψm, and O2- levels toward normal. We conclude that MB reversed NaCN-induced cardiotoxicity by preserving intracellular Ca2+ homeostasis and excitation-contraction coupling (ICa), minimizing risks of arrhythmias (Em, AP configuration, and depolarization-activated K+ currents), and reducing O2- levels. NEW & NOTEWORTHY Cyanide poisoning due to industrial exposure, smoke inhalation, and bioterrorism manifests as cardiogenic shock and requires rapidly effective antidote. In the early stage of cyanide exposure, adenosine triphosphate levels are normal but myocyte contractility is reduced, largely due to alterations in Ca2+ homeostasis because of changes in oxidation-reduction environment of ion channels. Methylene blue, a drug approved by the U.S. Food and Drug Administration, ameliorates cyanide toxicity by normalizing oxidation-reduction state and Ca2+ channel function.",
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Methylene blue counteracts cyanide cardiotoxicity : Cellular mechanisms. / Cheung, Joseph Y.; Wang, Jufang; Zhang, Xue Qian; Song, Jianliang; Tomar, Dhanendra; Madesh, Muniswamy; Judenherc-Haouzi, Annick; Haouzi, Philippe.

In: Journal of applied physiology, Vol. 124, No. 5, 05.2018, p. 1164-1176.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Methylene blue counteracts cyanide cardiotoxicity

T2 - Cellular mechanisms

AU - Cheung, Joseph Y.

AU - Wang, Jufang

AU - Zhang, Xue Qian

AU - Song, Jianliang

AU - Tomar, Dhanendra

AU - Madesh, Muniswamy

AU - Judenherc-Haouzi, Annick

AU - Haouzi, Philippe

PY - 2018/5

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N2 - In adult left ventricular mouse myocytes, exposure to sodium cyanide (NaCN) in the presence of glucose dose-dependently reduced contraction amplitude, with ∼80% of maximal inhibitory effect attained at 100 μM. NaCN (100 μM) exposure for 10 min significantly decreased contraction and intracellular Ca2+ concentration ([Ca2+]i) transient amplitudes, systolic but not diastolic [Ca2+]i, and maximal L-type Ca2+ current (ICa) amplitude, indicating acute alteration of [Ca2+]i homeostasis largely accounted for the observed excitation-contraction abnormalities. In addition, NaCN depolarized resting membrane potential (Em), reduced action potential (AP) amplitude, prolonged AP duration at 50% (APD50) and 90% repolarization (APD90), and suppressed depolarization-activated K+ currents but had no effect on Na+-Ca2+ exchange current (INaCa). NaCN did not affect cellular adenosine triphosphate levels but depolarized mitochondrial membrane potential (Δψm) and increased superoxide (O2-) levels. Methylene blue (MB; 20 μg/ml) added 3 min after NaCN restored contraction and [Ca2+]i transient amplitudes, systolic [Ca2+]i, Em, AP amplitude, APD50, APD90, ICa, depolarization-activated K+ currents, Δψm, and O2- levels toward normal. We conclude that MB reversed NaCN-induced cardiotoxicity by preserving intracellular Ca2+ homeostasis and excitation-contraction coupling (ICa), minimizing risks of arrhythmias (Em, AP configuration, and depolarization-activated K+ currents), and reducing O2- levels. NEW & NOTEWORTHY Cyanide poisoning due to industrial exposure, smoke inhalation, and bioterrorism manifests as cardiogenic shock and requires rapidly effective antidote. In the early stage of cyanide exposure, adenosine triphosphate levels are normal but myocyte contractility is reduced, largely due to alterations in Ca2+ homeostasis because of changes in oxidation-reduction environment of ion channels. Methylene blue, a drug approved by the U.S. Food and Drug Administration, ameliorates cyanide toxicity by normalizing oxidation-reduction state and Ca2+ channel function.

AB - In adult left ventricular mouse myocytes, exposure to sodium cyanide (NaCN) in the presence of glucose dose-dependently reduced contraction amplitude, with ∼80% of maximal inhibitory effect attained at 100 μM. NaCN (100 μM) exposure for 10 min significantly decreased contraction and intracellular Ca2+ concentration ([Ca2+]i) transient amplitudes, systolic but not diastolic [Ca2+]i, and maximal L-type Ca2+ current (ICa) amplitude, indicating acute alteration of [Ca2+]i homeostasis largely accounted for the observed excitation-contraction abnormalities. In addition, NaCN depolarized resting membrane potential (Em), reduced action potential (AP) amplitude, prolonged AP duration at 50% (APD50) and 90% repolarization (APD90), and suppressed depolarization-activated K+ currents but had no effect on Na+-Ca2+ exchange current (INaCa). NaCN did not affect cellular adenosine triphosphate levels but depolarized mitochondrial membrane potential (Δψm) and increased superoxide (O2-) levels. Methylene blue (MB; 20 μg/ml) added 3 min after NaCN restored contraction and [Ca2+]i transient amplitudes, systolic [Ca2+]i, Em, AP amplitude, APD50, APD90, ICa, depolarization-activated K+ currents, Δψm, and O2- levels toward normal. We conclude that MB reversed NaCN-induced cardiotoxicity by preserving intracellular Ca2+ homeostasis and excitation-contraction coupling (ICa), minimizing risks of arrhythmias (Em, AP configuration, and depolarization-activated K+ currents), and reducing O2- levels. NEW & NOTEWORTHY Cyanide poisoning due to industrial exposure, smoke inhalation, and bioterrorism manifests as cardiogenic shock and requires rapidly effective antidote. In the early stage of cyanide exposure, adenosine triphosphate levels are normal but myocyte contractility is reduced, largely due to alterations in Ca2+ homeostasis because of changes in oxidation-reduction environment of ion channels. Methylene blue, a drug approved by the U.S. Food and Drug Administration, ameliorates cyanide toxicity by normalizing oxidation-reduction state and Ca2+ channel function.

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