Mice expressing a "hyper-sensitive" form of the cannabinoid receptor 1 (CB1) are neither obese nor diabetic

David J. Marcus, Michael L. Zee, Brian J. Davis, Chris P. Haskins, Mary Jeanette Andrews, Randa Amin, Angela N. Henderson-Redmond, Ken Mackie, Traci A. Czyzyk, Daniel J. Morgan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Multiple lines of evidence implicate the endocannabinoid signaling system in the modulation of metabolic disease. Genetic or pharmacological inactivation of CB1 in rodents leads to reduced body weight, resistance to diet-induced obesity, decreased intake of highly palatable food, and increased energy expenditure. Cannabinoid agonists stimulate feeding in rodents and increased levels of endocannabinoids can disrupt lipid metabolism. Therefore, the hypothesis that sustained endocannabinoid signaling can lead to obesity and diabetes was examined in this study using S426A/S430A mutant mice expressing a desensitization-resistant CB1 receptor. These mice display exaggerated and prolonged responses to acute administration of phytocannabinoids, synthetic cannabinoids, and endocannabinoids. As a consequence these mice represent a novel model for determining the effect of enhanced endocannabinoid signaling on metabolic disease. S426A/S430A mutants consumed equivalent amounts of both high fat (45%) and low fat (10%) chow control diet compared to wildtype littermate controls. S426A/S430A mutants and wild-type mice fed either high or low fat control diet displayed similar fasting blood glucose levels and normal glucose clearance following a 2 g/kg glucose challenge. Furthermore, S426A/S430A mutants and wild-type mice consumed similar amounts of chow following an overnight fast. While both THC and JZL195 significantly increased food intake two hours after injection, this increase was similar between the S426A/S430A mutant and wildtype control mice Our results indicate that S426A/S430A mutant mice expressing the desensitization-resistant form of CB1 do not exhibit differences in body weight, food intake, glucose homeostasis, or re-feeding following a fast.

Original languageEnglish (US)
Article numbere0160462
JournalPloS one
Volume11
Issue number8
DOIs
StatePublished - Aug 2016

Fingerprint

Cannabinoid Receptors
Endocannabinoids
Nutrition
mice
mutants
Fats
Glucose
cannabinoids
Metabolic Diseases
metabolic diseases
Cannabinoid Receptor Agonists
glucose
food intake
Rodentia
Dronabinol
Cannabinoids
obesity
rodents
lipids
Obesity

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Marcus, David J. ; Zee, Michael L. ; Davis, Brian J. ; Haskins, Chris P. ; Andrews, Mary Jeanette ; Amin, Randa ; Henderson-Redmond, Angela N. ; Mackie, Ken ; Czyzyk, Traci A. ; Morgan, Daniel J. / Mice expressing a "hyper-sensitive" form of the cannabinoid receptor 1 (CB1) are neither obese nor diabetic. In: PloS one. 2016 ; Vol. 11, No. 8.
@article{3d24db5e362e46d99c394f5f07179c7e,
title = "Mice expressing a {"}hyper-sensitive{"} form of the cannabinoid receptor 1 (CB1) are neither obese nor diabetic",
abstract = "Multiple lines of evidence implicate the endocannabinoid signaling system in the modulation of metabolic disease. Genetic or pharmacological inactivation of CB1 in rodents leads to reduced body weight, resistance to diet-induced obesity, decreased intake of highly palatable food, and increased energy expenditure. Cannabinoid agonists stimulate feeding in rodents and increased levels of endocannabinoids can disrupt lipid metabolism. Therefore, the hypothesis that sustained endocannabinoid signaling can lead to obesity and diabetes was examined in this study using S426A/S430A mutant mice expressing a desensitization-resistant CB1 receptor. These mice display exaggerated and prolonged responses to acute administration of phytocannabinoids, synthetic cannabinoids, and endocannabinoids. As a consequence these mice represent a novel model for determining the effect of enhanced endocannabinoid signaling on metabolic disease. S426A/S430A mutants consumed equivalent amounts of both high fat (45{\%}) and low fat (10{\%}) chow control diet compared to wildtype littermate controls. S426A/S430A mutants and wild-type mice fed either high or low fat control diet displayed similar fasting blood glucose levels and normal glucose clearance following a 2 g/kg glucose challenge. Furthermore, S426A/S430A mutants and wild-type mice consumed similar amounts of chow following an overnight fast. While both THC and JZL195 significantly increased food intake two hours after injection, this increase was similar between the S426A/S430A mutant and wildtype control mice Our results indicate that S426A/S430A mutant mice expressing the desensitization-resistant form of CB1 do not exhibit differences in body weight, food intake, glucose homeostasis, or re-feeding following a fast.",
author = "Marcus, {David J.} and Zee, {Michael L.} and Davis, {Brian J.} and Haskins, {Chris P.} and Andrews, {Mary Jeanette} and Randa Amin and Henderson-Redmond, {Angela N.} and Ken Mackie and Czyzyk, {Traci A.} and Morgan, {Daniel J.}",
year = "2016",
month = "8",
doi = "10.1371/journal.pone.0160462",
language = "English (US)",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

Marcus, DJ, Zee, ML, Davis, BJ, Haskins, CP, Andrews, MJ, Amin, R, Henderson-Redmond, AN, Mackie, K, Czyzyk, TA & Morgan, DJ 2016, 'Mice expressing a "hyper-sensitive" form of the cannabinoid receptor 1 (CB1) are neither obese nor diabetic', PloS one, vol. 11, no. 8, e0160462. https://doi.org/10.1371/journal.pone.0160462

Mice expressing a "hyper-sensitive" form of the cannabinoid receptor 1 (CB1) are neither obese nor diabetic. / Marcus, David J.; Zee, Michael L.; Davis, Brian J.; Haskins, Chris P.; Andrews, Mary Jeanette; Amin, Randa; Henderson-Redmond, Angela N.; Mackie, Ken; Czyzyk, Traci A.; Morgan, Daniel J.

In: PloS one, Vol. 11, No. 8, e0160462, 08.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mice expressing a "hyper-sensitive" form of the cannabinoid receptor 1 (CB1) are neither obese nor diabetic

AU - Marcus, David J.

AU - Zee, Michael L.

AU - Davis, Brian J.

AU - Haskins, Chris P.

AU - Andrews, Mary Jeanette

AU - Amin, Randa

AU - Henderson-Redmond, Angela N.

AU - Mackie, Ken

AU - Czyzyk, Traci A.

AU - Morgan, Daniel J.

PY - 2016/8

Y1 - 2016/8

N2 - Multiple lines of evidence implicate the endocannabinoid signaling system in the modulation of metabolic disease. Genetic or pharmacological inactivation of CB1 in rodents leads to reduced body weight, resistance to diet-induced obesity, decreased intake of highly palatable food, and increased energy expenditure. Cannabinoid agonists stimulate feeding in rodents and increased levels of endocannabinoids can disrupt lipid metabolism. Therefore, the hypothesis that sustained endocannabinoid signaling can lead to obesity and diabetes was examined in this study using S426A/S430A mutant mice expressing a desensitization-resistant CB1 receptor. These mice display exaggerated and prolonged responses to acute administration of phytocannabinoids, synthetic cannabinoids, and endocannabinoids. As a consequence these mice represent a novel model for determining the effect of enhanced endocannabinoid signaling on metabolic disease. S426A/S430A mutants consumed equivalent amounts of both high fat (45%) and low fat (10%) chow control diet compared to wildtype littermate controls. S426A/S430A mutants and wild-type mice fed either high or low fat control diet displayed similar fasting blood glucose levels and normal glucose clearance following a 2 g/kg glucose challenge. Furthermore, S426A/S430A mutants and wild-type mice consumed similar amounts of chow following an overnight fast. While both THC and JZL195 significantly increased food intake two hours after injection, this increase was similar between the S426A/S430A mutant and wildtype control mice Our results indicate that S426A/S430A mutant mice expressing the desensitization-resistant form of CB1 do not exhibit differences in body weight, food intake, glucose homeostasis, or re-feeding following a fast.

AB - Multiple lines of evidence implicate the endocannabinoid signaling system in the modulation of metabolic disease. Genetic or pharmacological inactivation of CB1 in rodents leads to reduced body weight, resistance to diet-induced obesity, decreased intake of highly palatable food, and increased energy expenditure. Cannabinoid agonists stimulate feeding in rodents and increased levels of endocannabinoids can disrupt lipid metabolism. Therefore, the hypothesis that sustained endocannabinoid signaling can lead to obesity and diabetes was examined in this study using S426A/S430A mutant mice expressing a desensitization-resistant CB1 receptor. These mice display exaggerated and prolonged responses to acute administration of phytocannabinoids, synthetic cannabinoids, and endocannabinoids. As a consequence these mice represent a novel model for determining the effect of enhanced endocannabinoid signaling on metabolic disease. S426A/S430A mutants consumed equivalent amounts of both high fat (45%) and low fat (10%) chow control diet compared to wildtype littermate controls. S426A/S430A mutants and wild-type mice fed either high or low fat control diet displayed similar fasting blood glucose levels and normal glucose clearance following a 2 g/kg glucose challenge. Furthermore, S426A/S430A mutants and wild-type mice consumed similar amounts of chow following an overnight fast. While both THC and JZL195 significantly increased food intake two hours after injection, this increase was similar between the S426A/S430A mutant and wildtype control mice Our results indicate that S426A/S430A mutant mice expressing the desensitization-resistant form of CB1 do not exhibit differences in body weight, food intake, glucose homeostasis, or re-feeding following a fast.

UR - http://www.scopus.com/inward/record.url?scp=84983465347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983465347&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0160462

DO - 10.1371/journal.pone.0160462

M3 - Article

C2 - 27501235

AN - SCOPUS:84983465347

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e0160462

ER -