Micro-PIV measurements of the flow field around cells in flow chamber

Yi Fu, Jian Hua Wu, Jie Wu, Ren Sun, Zu Rong Ding, Cheng Dong

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

The velocity profile around cells in a flow chamber coated with the immobilized protein and the endothelial cells is studied using the micro particle image velocimetry (PIV). The main purpose is to study the effect of the endothelial cells on the local hydrodynamic environment and the local shear rates above a single polymorphonuclear neutrophil (PMN) and a melanoma cell when they adhere to different immobilized protein substrates. Micro-PIV images are taken in the top-view and the side-view under 10X and 40X objective lens and the ensemble correlation method is used to analyze the data. The results show that the endothelial monolayer has changed the patterns of the flow velocity profile of the side-view flow on the chamber bottom, and also increased the wall shear rates. The melanoma cells adhered on the immobilized fibrin disturb the local flow more than those adhered on the immobilized fibrinogen, but one sees no significant difference between the local shear rates above the PMNs adhered on the immobilized fibrinogen and those above the PMNs adhered on the immobilized fibrin.

Original languageEnglish (US)
Pages (from-to)562-568
Number of pages7
JournalJournal of Hydrodynamics
Volume27
Issue number4
DOIs
StatePublished - Oct 1 2015

Fingerprint

flow chambers
particle image velocimetry
fibrin
Velocity measurement
Shear deformation
Flow Field
fibrinogen
Flow fields
flow distribution
Endothelial cells
shear
Endothelial Cells
Melanoma
Cell
Velocity Profile
velocity distribution
cells
neutrophils
proteins
Proteins

All Science Journal Classification (ASJC) codes

  • Modeling and Simulation
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Fu, Yi ; Wu, Jian Hua ; Wu, Jie ; Sun, Ren ; Ding, Zu Rong ; Dong, Cheng. / Micro-PIV measurements of the flow field around cells in flow chamber. In: Journal of Hydrodynamics. 2015 ; Vol. 27, No. 4. pp. 562-568.
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abstract = "The velocity profile around cells in a flow chamber coated with the immobilized protein and the endothelial cells is studied using the micro particle image velocimetry (PIV). The main purpose is to study the effect of the endothelial cells on the local hydrodynamic environment and the local shear rates above a single polymorphonuclear neutrophil (PMN) and a melanoma cell when they adhere to different immobilized protein substrates. Micro-PIV images are taken in the top-view and the side-view under 10X and 40X objective lens and the ensemble correlation method is used to analyze the data. The results show that the endothelial monolayer has changed the patterns of the flow velocity profile of the side-view flow on the chamber bottom, and also increased the wall shear rates. The melanoma cells adhered on the immobilized fibrin disturb the local flow more than those adhered on the immobilized fibrinogen, but one sees no significant difference between the local shear rates above the PMNs adhered on the immobilized fibrinogen and those above the PMNs adhered on the immobilized fibrin.",
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Micro-PIV measurements of the flow field around cells in flow chamber. / Fu, Yi; Wu, Jian Hua; Wu, Jie; Sun, Ren; Ding, Zu Rong; Dong, Cheng.

In: Journal of Hydrodynamics, Vol. 27, No. 4, 01.10.2015, p. 562-568.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Micro-PIV measurements of the flow field around cells in flow chamber

AU - Fu, Yi

AU - Wu, Jian Hua

AU - Wu, Jie

AU - Sun, Ren

AU - Ding, Zu Rong

AU - Dong, Cheng

PY - 2015/10/1

Y1 - 2015/10/1

N2 - The velocity profile around cells in a flow chamber coated with the immobilized protein and the endothelial cells is studied using the micro particle image velocimetry (PIV). The main purpose is to study the effect of the endothelial cells on the local hydrodynamic environment and the local shear rates above a single polymorphonuclear neutrophil (PMN) and a melanoma cell when they adhere to different immobilized protein substrates. Micro-PIV images are taken in the top-view and the side-view under 10X and 40X objective lens and the ensemble correlation method is used to analyze the data. The results show that the endothelial monolayer has changed the patterns of the flow velocity profile of the side-view flow on the chamber bottom, and also increased the wall shear rates. The melanoma cells adhered on the immobilized fibrin disturb the local flow more than those adhered on the immobilized fibrinogen, but one sees no significant difference between the local shear rates above the PMNs adhered on the immobilized fibrinogen and those above the PMNs adhered on the immobilized fibrin.

AB - The velocity profile around cells in a flow chamber coated with the immobilized protein and the endothelial cells is studied using the micro particle image velocimetry (PIV). The main purpose is to study the effect of the endothelial cells on the local hydrodynamic environment and the local shear rates above a single polymorphonuclear neutrophil (PMN) and a melanoma cell when they adhere to different immobilized protein substrates. Micro-PIV images are taken in the top-view and the side-view under 10X and 40X objective lens and the ensemble correlation method is used to analyze the data. The results show that the endothelial monolayer has changed the patterns of the flow velocity profile of the side-view flow on the chamber bottom, and also increased the wall shear rates. The melanoma cells adhered on the immobilized fibrin disturb the local flow more than those adhered on the immobilized fibrinogen, but one sees no significant difference between the local shear rates above the PMNs adhered on the immobilized fibrinogen and those above the PMNs adhered on the immobilized fibrin.

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