Micro sensors: Linking real-time oscillatory shear stress with vascular inflammatory responses

Tzung K. Hsiai, Sung K. Cho, Pak Kin Wong, Michael H. Ing, Adler Salazar, Susan Hama, Mohamad Navab, Linda L. Demer, Chih Ming Ho

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The important interplay between blood circulation and vascular cell behavior warrants the development of highly sensitive but small sensing systems. The emerging micro electro mechanical systems (MEMS) technology, thus, provides the high spatiotemporal resolution to link biomechanical forces on the microscale with large-scale physiology. We fabricated MEMS sensors, comparable to the endothelial cells (ECs) in size, to link real-time shear stress with monocyte/EC interactions in an oscillatory flow environment, simulating the moving and unsteady separation point at arterial bifurcations. In response to oscillatory shear stress (τ) at ± 2.6 dyn/cm 2, time-averaged shear stress (τ ave) = 0 at 0.5 Hz, individual monocytes displayed unique to-and-fro trajectories, undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Incorporating with cell-tracking velocimetry, we visualized that these real-time events occurred over a dynamic range of oscillating shear stress between ±2.6 dyn/cm 2 and Reynolds number between 0 and 22.2 in the presence of activated adhesion molecule and chemokine mRNA expression.

Original languageEnglish (US)
Pages (from-to)189-201
Number of pages13
JournalAnnals of Biomedical Engineering
Volume32
Issue number2
DOIs
StatePublished - Feb 1 2004

Fingerprint

Shear stress
Endothelial cells
Sensors
Adhesion
Physiology
Hemodynamics
Velocity measurement
Reynolds number
Trajectories
Molecules

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Cite this

Hsiai, Tzung K. ; Cho, Sung K. ; Wong, Pak Kin ; Ing, Michael H. ; Salazar, Adler ; Hama, Susan ; Navab, Mohamad ; Demer, Linda L. ; Ho, Chih Ming. / Micro sensors : Linking real-time oscillatory shear stress with vascular inflammatory responses. In: Annals of Biomedical Engineering. 2004 ; Vol. 32, No. 2. pp. 189-201.
@article{a5f037424a5f45469e22216f4bdbf186,
title = "Micro sensors: Linking real-time oscillatory shear stress with vascular inflammatory responses",
abstract = "The important interplay between blood circulation and vascular cell behavior warrants the development of highly sensitive but small sensing systems. The emerging micro electro mechanical systems (MEMS) technology, thus, provides the high spatiotemporal resolution to link biomechanical forces on the microscale with large-scale physiology. We fabricated MEMS sensors, comparable to the endothelial cells (ECs) in size, to link real-time shear stress with monocyte/EC interactions in an oscillatory flow environment, simulating the moving and unsteady separation point at arterial bifurcations. In response to oscillatory shear stress (τ) at ± 2.6 dyn/cm 2, time-averaged shear stress (τ ave) = 0 at 0.5 Hz, individual monocytes displayed unique to-and-fro trajectories, undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Incorporating with cell-tracking velocimetry, we visualized that these real-time events occurred over a dynamic range of oscillating shear stress between ±2.6 dyn/cm 2 and Reynolds number between 0 and 22.2 in the presence of activated adhesion molecule and chemokine mRNA expression.",
author = "Hsiai, {Tzung K.} and Cho, {Sung K.} and Wong, {Pak Kin} and Ing, {Michael H.} and Adler Salazar and Susan Hama and Mohamad Navab and Demer, {Linda L.} and Ho, {Chih Ming}",
year = "2004",
month = "2",
day = "1",
doi = "10.1023/B:ABME.0000012739.88554.01",
language = "English (US)",
volume = "32",
pages = "189--201",
journal = "Annals of Biomedical Engineering",
issn = "0090-6964",
publisher = "Springer Netherlands",
number = "2",

}

Micro sensors : Linking real-time oscillatory shear stress with vascular inflammatory responses. / Hsiai, Tzung K.; Cho, Sung K.; Wong, Pak Kin; Ing, Michael H.; Salazar, Adler; Hama, Susan; Navab, Mohamad; Demer, Linda L.; Ho, Chih Ming.

In: Annals of Biomedical Engineering, Vol. 32, No. 2, 01.02.2004, p. 189-201.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Micro sensors

T2 - Linking real-time oscillatory shear stress with vascular inflammatory responses

AU - Hsiai, Tzung K.

AU - Cho, Sung K.

AU - Wong, Pak Kin

AU - Ing, Michael H.

AU - Salazar, Adler

AU - Hama, Susan

AU - Navab, Mohamad

AU - Demer, Linda L.

AU - Ho, Chih Ming

PY - 2004/2/1

Y1 - 2004/2/1

N2 - The important interplay between blood circulation and vascular cell behavior warrants the development of highly sensitive but small sensing systems. The emerging micro electro mechanical systems (MEMS) technology, thus, provides the high spatiotemporal resolution to link biomechanical forces on the microscale with large-scale physiology. We fabricated MEMS sensors, comparable to the endothelial cells (ECs) in size, to link real-time shear stress with monocyte/EC interactions in an oscillatory flow environment, simulating the moving and unsteady separation point at arterial bifurcations. In response to oscillatory shear stress (τ) at ± 2.6 dyn/cm 2, time-averaged shear stress (τ ave) = 0 at 0.5 Hz, individual monocytes displayed unique to-and-fro trajectories, undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Incorporating with cell-tracking velocimetry, we visualized that these real-time events occurred over a dynamic range of oscillating shear stress between ±2.6 dyn/cm 2 and Reynolds number between 0 and 22.2 in the presence of activated adhesion molecule and chemokine mRNA expression.

AB - The important interplay between blood circulation and vascular cell behavior warrants the development of highly sensitive but small sensing systems. The emerging micro electro mechanical systems (MEMS) technology, thus, provides the high spatiotemporal resolution to link biomechanical forces on the microscale with large-scale physiology. We fabricated MEMS sensors, comparable to the endothelial cells (ECs) in size, to link real-time shear stress with monocyte/EC interactions in an oscillatory flow environment, simulating the moving and unsteady separation point at arterial bifurcations. In response to oscillatory shear stress (τ) at ± 2.6 dyn/cm 2, time-averaged shear stress (τ ave) = 0 at 0.5 Hz, individual monocytes displayed unique to-and-fro trajectories, undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Incorporating with cell-tracking velocimetry, we visualized that these real-time events occurred over a dynamic range of oscillating shear stress between ±2.6 dyn/cm 2 and Reynolds number between 0 and 22.2 in the presence of activated adhesion molecule and chemokine mRNA expression.

UR - http://www.scopus.com/inward/record.url?scp=2942558759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942558759&partnerID=8YFLogxK

U2 - 10.1023/B:ABME.0000012739.88554.01

DO - 10.1023/B:ABME.0000012739.88554.01

M3 - Article

C2 - 15008367

AN - SCOPUS:2942558759

VL - 32

SP - 189

EP - 201

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

SN - 0090-6964

IS - 2

ER -