Microbial ATP bioluminescence as a means to detect contamination on artificially contaminated beef carcass tissue

Gregory R. Siragusa, Catherine Nettles Cutter

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The use of microbial ATP bioluminescence was evaluated as a means to rapidly detect gross microbial contamination from feces on bovine-carcass surface tissue (BCT). Microbial ATP was selectively distinguished from nonmicrobial ATP by the assay procedure used. Regression analyses of microbial ATP and viable count scatterplots showed lean and adipose BCT artificially contaminated with bovine feces had the same regression line parameters (P < 0.05), and therefore, the microbial ATP responses were similar for both tissue types. Correlation coefficients (r) of these regression lines were >0.90 for both tissue types. Results indicated that swab samples can be held at 5°C for up to 6 h without compromising microbial ATP bioluminescence assay results. The microbial ATP bioluminescence assay shows potential for use as a means to rapidly detect fecal contamination on red meat carcasses and to gauge decontamination effectiveness and hence could monitor critical control points in a processing-plant HACCP plan.

Original languageEnglish (US)
Pages (from-to)764-769
Number of pages6
JournalJournal of Food Protection
Volume58
Issue number7
DOIs
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

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