Microbial reduction of U(VI) at the solid-water interface

Byong Hun Jeon, Shelly D. Kelly, Kenneth M. Kemner, Mark O. Barnett, William D. Burgos, Brian A. Dempsey, Eric E. Roden

Research output: Contribution to journalArticle

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Abstract

Microbial (Geobacter sulfurreducens) reduction of 0.1 mM U(VI) in the presence of synthetic Fe(III) oxides and natural Fe(III) oxide-containing solids was investigated in pH 6.8 artificial groundwater containing 10 mM NaHCO 3. In most experiments, more than 95% of added U(VI) was sorbed to solids, so that U(VI) reduction was governed by reactions at the solid-water interface. The rate and extent of reduction of U(VI) associated with surfaces of synthetic Fe(III) oxides (hydrous ferric oxide, goethite, and hematite) was comparable to that observed during reduction of aqueous U(VI). In contrast, microbial reduction of U(VI) sorbed to several different natural Fe(III) oxide-containing solids was slower and less extensive compared to synthetic Fe(III) oxide systems. Addition of the electron shuttling agent anthraquinone-2,6-disulfonate (AQDS; 0.1 mM) enhanced the rate and extent of both Fe(III) and U(VI) reduction. These findings suggest that AQDS facilitated electron transfer from G. sulfurreducens to U(VI) associated with surface sites atwhich direct enzymatic reduction was kinetically limited. Our results demonstrate that association of U(VI) with diverse surface sites in natural soils and sediments has the potential to limit the rate and extent of microbial U(VI) reduction and thereby modulate the effectiveness of in situ U(VI) bioremediation.

Original languageEnglish (US)
Pages (from-to)5649-5655
Number of pages7
JournalEnvironmental Science and Technology
Volume38
Issue number21
DOIs
StatePublished - Nov 1 2004

Fingerprint

Water
oxide
Oxides
water
electron
Electrons
Bioremediation
Hematite
goethite
bioremediation
hematite
Groundwater
Sediments
Association reactions
Soils
groundwater
sediment
rate
soil
experiment

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Jeon, B. H., Kelly, S. D., Kemner, K. M., Barnett, M. O., Burgos, W. D., Dempsey, B. A., & Roden, E. E. (2004). Microbial reduction of U(VI) at the solid-water interface. Environmental Science and Technology, 38(21), 5649-5655. https://doi.org/10.1021/es0496120
Jeon, Byong Hun ; Kelly, Shelly D. ; Kemner, Kenneth M. ; Barnett, Mark O. ; Burgos, William D. ; Dempsey, Brian A. ; Roden, Eric E. / Microbial reduction of U(VI) at the solid-water interface. In: Environmental Science and Technology. 2004 ; Vol. 38, No. 21. pp. 5649-5655.
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Jeon, BH, Kelly, SD, Kemner, KM, Barnett, MO, Burgos, WD, Dempsey, BA & Roden, EE 2004, 'Microbial reduction of U(VI) at the solid-water interface', Environmental Science and Technology, vol. 38, no. 21, pp. 5649-5655. https://doi.org/10.1021/es0496120

Microbial reduction of U(VI) at the solid-water interface. / Jeon, Byong Hun; Kelly, Shelly D.; Kemner, Kenneth M.; Barnett, Mark O.; Burgos, William D.; Dempsey, Brian A.; Roden, Eric E.

In: Environmental Science and Technology, Vol. 38, No. 21, 01.11.2004, p. 5649-5655.

Research output: Contribution to journalArticle

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T1 - Microbial reduction of U(VI) at the solid-water interface

AU - Jeon, Byong Hun

AU - Kelly, Shelly D.

AU - Kemner, Kenneth M.

AU - Barnett, Mark O.

AU - Burgos, William D.

AU - Dempsey, Brian A.

AU - Roden, Eric E.

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AB - Microbial (Geobacter sulfurreducens) reduction of 0.1 mM U(VI) in the presence of synthetic Fe(III) oxides and natural Fe(III) oxide-containing solids was investigated in pH 6.8 artificial groundwater containing 10 mM NaHCO 3. In most experiments, more than 95% of added U(VI) was sorbed to solids, so that U(VI) reduction was governed by reactions at the solid-water interface. The rate and extent of reduction of U(VI) associated with surfaces of synthetic Fe(III) oxides (hydrous ferric oxide, goethite, and hematite) was comparable to that observed during reduction of aqueous U(VI). In contrast, microbial reduction of U(VI) sorbed to several different natural Fe(III) oxide-containing solids was slower and less extensive compared to synthetic Fe(III) oxide systems. Addition of the electron shuttling agent anthraquinone-2,6-disulfonate (AQDS; 0.1 mM) enhanced the rate and extent of both Fe(III) and U(VI) reduction. These findings suggest that AQDS facilitated electron transfer from G. sulfurreducens to U(VI) associated with surface sites atwhich direct enzymatic reduction was kinetically limited. Our results demonstrate that association of U(VI) with diverse surface sites in natural soils and sediments has the potential to limit the rate and extent of microbial U(VI) reduction and thereby modulate the effectiveness of in situ U(VI) bioremediation.

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Jeon BH, Kelly SD, Kemner KM, Barnett MO, Burgos WD, Dempsey BA et al. Microbial reduction of U(VI) at the solid-water interface. Environmental Science and Technology. 2004 Nov 1;38(21):5649-5655. https://doi.org/10.1021/es0496120