Microbiota fermentation-NLRP3 axis shapes the impact of dietary fibres on intestinal inflammation

Vishal Singh, Beng San Yeoh, Rachel E. Walker, Xia Xiao, Piu Saha, Rachel M. Golonka, Jingwei Cai, Alexis Charles Andre Bretin, Xi Cheng, Qing Liu, Michael D. Flythe, Benoit Chassaing, Gregory C. Shearer, Andrew D. Patterson, Andrew T. Gewirtz, Matam Vijay-Kumar

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Objective Diets rich in fermentable fibres provide an array of health benefits; however, many patients with IBD report poor tolerance to fermentable fibre-rich foods. Intervention studies with dietary fibres in murine models of colonic inflammation have yielded conflicting results on whether fibres ameliorate or exacerbate IBD. Herein, we examined how replacing the insoluble fibre, cellulose, with the fermentable fibres, inulin or pectin, impacted murine colitis resulting from immune dysregulation via inhibition of interleukin (IL)-10 signalling and/or innate immune deficiency (Tlr5KO). Design Mice were fed with diet containing either cellulose, inulin or pectin and subjected to weekly injections of an IL-10 receptor (αIL-10R) neutralising antibody. Colitis development was examined by serological, biochemical, histological and immunological parameters. Results Inulin potentiated the severity of αIL10R-induced colitis, while pectin ameliorated the disease. Such exacerbation of colitis following inulin feeding was associated with enrichment of butyrate-producing bacteria and elevated levels of caecal butyrate. Blockade of butyrate production by either metronidazole or hops β-acids ameliorated colitis severity in inulin-fed mice, whereas augmenting caecal butyrate via tributyrin increased colitis severity in cellulose containing diet-fed mice. Elevated butyrate levels were associated with increased IL-1β activity, while inhibition of the NOD-like receptor protein 3 by genetic, pharmacologic or dietary means markedly reduced colitis. Conclusion These results not only support the notion that fermentable fibres have the potential to ameliorate colitis but also caution that, in some contexts, prebiotic fibres can lead to gut dysbiosis and surfeit colonic butyrate that might exacerbate IBD.

Original languageEnglish (US)
Pages (from-to)1801-1812
Number of pages12
JournalGut
Volume68
Issue number10
DOIs
StatePublished - Oct 1 2019

All Science Journal Classification (ASJC) codes

  • Gastroenterology

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