Microcalorimetric detection of creatinine in urine

David E. Gaddes, Srinivas A. Tadigadapa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we demonstrate the quantification of creatinine in human urine samples using a micro-calorimetric sensing system. The calorimetric sensor is based on an array of microfabricated Y-cut quartz resonators. The piezoelectric quartz is etched down to a thickness of 10 μm and exhibits a bulk acoustic resonance of 166 MHz. The temperature sensitivity of this high-frequency quartz resonator is 14,600 Hz/K due to the high phenomenological sensitivity of quartz. The sensing resonator structure is thermally isolated from the bulk quartz by fabricating a plate-like cantilevered structure from the etched quartz using focused ion beam etching. Importantly, the quartz sensors and fluidics are decoupled providing a significantly more robust calorimetric sensing system than directly contacted thermopile based chip calorimeters. We employ alginate entrapped creatinine deiminase to transduce urinary creatinine into temperature signatures allowing for the quantification. Ultimately, we envision the development of a handheld calorimetric device similar to that of the glucose meter but with the capability of detecting a much wider range of biomolecules due to the near universality of calorimetric sensing.

Original languageEnglish (US)
Title of host publicationIEEE Sensors, SENSORS 2016 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479982875
DOIs
StatePublished - Jan 5 2017
Event15th IEEE Sensors Conference, SENSORS 2016 - Orlando, United States
Duration: Oct 30 2016Nov 2 2016

Publication series

NameProceedings of IEEE Sensors
ISSN (Print)1930-0395
ISSN (Electronic)2168-9229

Other

Other15th IEEE Sensors Conference, SENSORS 2016
CountryUnited States
CityOrlando
Period10/30/1611/2/16

Fingerprint

Quartz
Resonators
Thermopiles
Focused ion beams
Alginate
Sensors
Fluidics
Biomolecules
Calorimeters
Glucose
Etching
Acoustics
Temperature

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Gaddes, D. E., & Tadigadapa, S. A. (2017). Microcalorimetric detection of creatinine in urine. In IEEE Sensors, SENSORS 2016 - Proceedings [7808469] (Proceedings of IEEE Sensors). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2016.7808469
Gaddes, David E. ; Tadigadapa, Srinivas A. / Microcalorimetric detection of creatinine in urine. IEEE Sensors, SENSORS 2016 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. (Proceedings of IEEE Sensors).
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Gaddes, DE & Tadigadapa, SA 2017, Microcalorimetric detection of creatinine in urine. in IEEE Sensors, SENSORS 2016 - Proceedings., 7808469, Proceedings of IEEE Sensors, Institute of Electrical and Electronics Engineers Inc., 15th IEEE Sensors Conference, SENSORS 2016, Orlando, United States, 10/30/16. https://doi.org/10.1109/ICSENS.2016.7808469

Microcalorimetric detection of creatinine in urine. / Gaddes, David E.; Tadigadapa, Srinivas A.

IEEE Sensors, SENSORS 2016 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. 7808469 (Proceedings of IEEE Sensors).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Gaddes DE, Tadigadapa SA. Microcalorimetric detection of creatinine in urine. In IEEE Sensors, SENSORS 2016 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2017. 7808469. (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2016.7808469