Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy

Michael A. Casasanta, G. M. Jonaid, Liam Kaylor, William Y. Luqiu, Maria J. Solares, Mariah L. Schroen, William J. Dearnaley, Jarad Wilson, Madeline J. Dukes, Deborah F. Kelly

Research output: Contribution to journalArticlepeer-review

Abstract

Interest in cryo-Electron Microscopy (EM) imaging has skyrocketed in recent years due to its pristine views of macromolecules and materials. As advances in instrumentation and computing algorithms spurred this progress, there is renewed focus to address specimen-related challenges. Here we contribute a microchip-based toolkit to perform complementary structural and biochemical analysis on low-molecular weight proteins. As a model system, we used the SARS-CoV-2 nucleocapsid (N) protein (48 kDa) due to its stability and important role in therapeutic development. Cryo-EM structures of the N protein monomer revealed a flexible N-terminal "top hat"motif and a helical-rich C-terminal domain. To complement our structural findings, we engineered microchip-based immunoprecipitation assays that led to the discovery of the first antibody binding site on the N protein. The data also facilitated molecular modeling of a variety of pandemic and common cold-related coronavirus proteins. Such insights may guide future pandemic-preparedness protocols through immuno-engineering strategies to mitigate viral outbreaks. This journal is

Original languageEnglish (US)
Pages (from-to)7285-7293
Number of pages9
JournalNanoscale
Volume13
Issue number15
DOIs
StatePublished - Apr 21 2021

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

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