Microcystin-LR-induced Ultrastructural Changes in Rats

S. B. Hooser, Val Richard Beasley, E. J. Basgall, W. W. Carmichael, W. M. Haschek

Research output: Contribution to journalArticle

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Abstract

The ultrastructure of hepatic, pulmonary, and renal lesions was evaluated in rats injected intraperitoneally with a lethal dose of microcystin-LR (MCLR, 160 μg/kg), a cyclic heptapeptide hepatotoxin produced by the blue-green algae, Microcystis aeruginosa. Hepatic lesions were first Seen at 10 minutes post-dosing and consisted of mild widening of hepatocyte intercellular spaces centrilobularly. At 20 minutes post-dosing, hepatocyte plasma membrane alterations were more pronounced, consisting of plasma membrane invagination with formation of variably sized and shaped intracytoplasmic vacuoles, loss of microvilli along the sinusoidal face, and widespread, pronounced hepatocyte separation. By 30 minutes, the space of Disse was markedly widened. At 60 minutes post-dosing, centrilobular areas contained necrotic cells and apparently intact, isolated, organelles intermingled with erythrocytes and platelets. In less severely affected regions there was prominent hepatocyte rounding, and erythrocytes and platelets were present in the widened space of Disse. Large amounts of hepatocellular debris and intact hepatocytes were present in the pulmonary vasculature, while smaller amounts of debris were also seen in the glomerular and peritubular capillaries of the renal cortex. This study shows that initial lesions are confined to Shape changes in the plasma membrane of hepatocytes. These changes are consistent with the hypothesis that microcystin-LR induces alterations in the hepatocyte cytoskeleton. Later changes consist of hepatocyte disassociation and necrosis, as well as endothelial damage, which allow release of hepatocytes and debris into the circulation with microembolism in lungs and kidneys.

Original languageEnglish (US)
Pages (from-to)9-15
Number of pages7
JournalVeterinary Pathology
Volume27
Issue number1
DOIs
StatePublished - Jan 1 1990

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microcystin-LR
hepatocytes
Hepatocytes
rats
lesions (animal)
plasma membrane
lungs
kidneys
Cell Membrane
Kidney
Lung
erythrocytes
Blood Platelets
Erythrocytes
Microcystis
hepatotoxins
cyanoginosin LR
liver
Microcystis aeruginosa
intercellular spaces

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Hooser, S. B., Beasley, V. R., Basgall, E. J., Carmichael, W. W., & Haschek, W. M. (1990). Microcystin-LR-induced Ultrastructural Changes in Rats. Veterinary Pathology, 27(1), 9-15. https://doi.org/10.1177/030098589002700102
Hooser, S. B. ; Beasley, Val Richard ; Basgall, E. J. ; Carmichael, W. W. ; Haschek, W. M. / Microcystin-LR-induced Ultrastructural Changes in Rats. In: Veterinary Pathology. 1990 ; Vol. 27, No. 1. pp. 9-15.
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Hooser, SB, Beasley, VR, Basgall, EJ, Carmichael, WW & Haschek, WM 1990, 'Microcystin-LR-induced Ultrastructural Changes in Rats', Veterinary Pathology, vol. 27, no. 1, pp. 9-15. https://doi.org/10.1177/030098589002700102

Microcystin-LR-induced Ultrastructural Changes in Rats. / Hooser, S. B.; Beasley, Val Richard; Basgall, E. J.; Carmichael, W. W.; Haschek, W. M.

In: Veterinary Pathology, Vol. 27, No. 1, 01.01.1990, p. 9-15.

Research output: Contribution to journalArticle

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