Microfluidic cell co-culture using standing surface acoustic wave (SSAW)

Sixing Li, Feng Guo, Yuchao Chen, Xiaoyun Ding, Peng Li, Craig E. Cameron, Tony Jun Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The in vitro study of heterotypic cell-cell interactions requires co-culturing different types of cells in a highly controllable manner. In this work, we demonstrate a standing surface acoustic wave (SSAW)-based microfluidic device that is capable of patterning different types of cells sequentially in the generated SSAW field to form organized cell co-cultures. In our approach, non-invasive acoustic forces are utilized to manipulate cells in the SSAW field. The advantages of our SSAW-based microfluidic cell co-culture platform include contactless cell manipulation, simplicity, high biocompatibility, high resolution, and minimal interference of the cellular microenvironment. The microfluidic platform demonstrated here can be a valuable tool for studying cell-cell interactions and multicellular tissue reconstruction.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages533-535
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

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All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Li, S., Guo, F., Chen, Y., Ding, X., Li, P., Cameron, C. E., & Huang, T. J. (2014). Microfluidic cell co-culture using standing surface acoustic wave (SSAW). In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 533-535). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.