Microfluidic cell surface antigen expression analysis using a single antibody type

Ye Zhang, Dimitri Pappas

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Antigen expression plays a significant role in clinical studies, pathology, biology and chemistry. The type and degree of antigen expression can provide information for disease diagnosis/monitoring and is used for phenotype analysis of cells. In this work, an affinity capture method was developed to capture cells based on antigen expression differences in a single microfluidic chip. Microfluidic chips with two affinity regions - at different antibody concentrations - captured two cell types based on differences in the expression of a single antigen. Using herringbone-modified capture channels, a separation purity of 95% and a capture efficiency of 15% were achieved under continuous-flow conditions. We observed that the capture ratio of Ramos B lymphocytes and HuT 78 T lymphocytes matched the expression ratio of CD71 for the two cell lines (R2 = 0.94). To further validate our analytical method, Ramos B lymphocytes were spiked into blood samples to demonstrate performance with a complex sample. Expression ratios matched conventional flow cytometry measurements over a 40-fold difference, and the sample enrichment was 9.5×. This method has proven to be a robust system to measure the differences in antigen expression, and can be used to distinguish cells without having a unique surface antigen if the expression level is sufficiently high in one cell type.

Original languageEnglish (US)
Pages (from-to)1440-1447
Number of pages8
JournalAnalyst
Volume141
Issue number4
DOIs
StatePublished - Feb 21 2016

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Microfluidics
Surface Antigens
Antigens
antigen
Antibodies
antibody
Lymphocytes
B-Lymphocytes
Clinical Chemistry
Clinical Pathology
T-cells
Flow cytometry
capture method
Pathology
flow cytometry
pathology
Flow Cytometry
Blood
Cells
analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Zhang, Ye ; Pappas, Dimitri. / Microfluidic cell surface antigen expression analysis using a single antibody type. In: Analyst. 2016 ; Vol. 141, No. 4. pp. 1440-1447.
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Microfluidic cell surface antigen expression analysis using a single antibody type. / Zhang, Ye; Pappas, Dimitri.

In: Analyst, Vol. 141, No. 4, 21.02.2016, p. 1440-1447.

Research output: Contribution to journalArticle

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