Microfluidic device and system for point-of-care blood coagulation measurement based on electrical impedance sensing

Bharath Ramaswamy, Yin-Ting Yeh, Siyang Zheng

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Blood coagulation tests are an integral part of diagnosis and treatment of patients with cardiac disorders. The activated partial thromboplastin time test (aPTT or APTT) is performed on a large scale, in laboratories, to monitor the functioning of the intrinsic and common pathways of the blood coagulation cascade and the concentration of anticoagulants such as heparin among patients. A polydimethylsiloxane (PDMS) based microfluidic device has been fabricated and tested to perform APTT as a point-of-care device using whole blood samples by detecting the change in electrical impedance of blood during coagulation. A portable setup consisting of the device and a lock-in amplifier circuit was built and tested for repeatability and stand alone usage. A frequency sweep test was performed on the device and the optimal frequency of operation for maximum output sensitivity was determined. The devices were also tested for their sensitivity to different concentrations of heparin and found to exhibit an expected increase in coagulation time for increasing heparin concentration.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume180
DOIs
StatePublished - Apr 1 2013

Fingerprint

blood coagulation
Acoustic impedance
microfluidic devices
electrical impedance
heparins
Coagulation
Microfluidics
Blood
Heparin
thromboplastin
anticoagulants
sweep frequency
sensitivity
Thromboplastin
Polydimethylsiloxane
coagulation
Anticoagulants
blood
cascades
amplifiers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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abstract = "Blood coagulation tests are an integral part of diagnosis and treatment of patients with cardiac disorders. The activated partial thromboplastin time test (aPTT or APTT) is performed on a large scale, in laboratories, to monitor the functioning of the intrinsic and common pathways of the blood coagulation cascade and the concentration of anticoagulants such as heparin among patients. A polydimethylsiloxane (PDMS) based microfluidic device has been fabricated and tested to perform APTT as a point-of-care device using whole blood samples by detecting the change in electrical impedance of blood during coagulation. A portable setup consisting of the device and a lock-in amplifier circuit was built and tested for repeatability and stand alone usage. A frequency sweep test was performed on the device and the optimal frequency of operation for maximum output sensitivity was determined. The devices were also tested for their sensitivity to different concentrations of heparin and found to exhibit an expected increase in coagulation time for increasing heparin concentration.",
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