Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood

B. Ramaswamy, Yin-Ting Yeh, Siyang Zheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The Activated Partial Thromboplastin Time (APTT) test is performed on a large scale, in laboratories to monitor the functioning of intrinsic and common pathways of blood coagulation cascade and the concentration of anticoagulants such as Heparin among patients. A PDMS based microfluidic device has been fabricated and tested to perform APTT as a point-of-care device using whole blood samples by detecting the change in electrical impedance of blood during coagulation. The two chambered PDMS device integrated with microelectrodes is pre-coated with APTT reagent and heparin respectively for better repeatability. The results indicate a repeatable pattern of change in whole blood impedance during coagulation and confirm the theoretical explanation by Hartert [1]. The devices were also tested for their sensitivity to different concentrations of heparin and found to exhibit a favorable increase in coagulation time for increasing heparin concentration.

Original languageEnglish (US)
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages222-225
Number of pages4
DOIs
StatePublished - Sep 1 2011
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: Jun 5 2011Jun 9 2011

Other

Other2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CountryChina
CityBeijing
Period6/5/116/9/11

Fingerprint

Coagulation
Microfluidics
Blood
Acoustic impedance
Microelectrodes
Heparin

All Science Journal Classification (ASJC) codes

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Ramaswamy, B., Yeh, Y-T., & Zheng, S. (2011). Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 222-225). [5969391] https://doi.org/10.1109/TRANSDUCERS.2011.5969391
Ramaswamy, B. ; Yeh, Yin-Ting ; Zheng, Siyang. / Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood. 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. pp. 222-225
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abstract = "The Activated Partial Thromboplastin Time (APTT) test is performed on a large scale, in laboratories to monitor the functioning of intrinsic and common pathways of blood coagulation cascade and the concentration of anticoagulants such as Heparin among patients. A PDMS based microfluidic device has been fabricated and tested to perform APTT as a point-of-care device using whole blood samples by detecting the change in electrical impedance of blood during coagulation. The two chambered PDMS device integrated with microelectrodes is pre-coated with APTT reagent and heparin respectively for better repeatability. The results indicate a repeatable pattern of change in whole blood impedance during coagulation and confirm the theoretical explanation by Hartert [1]. The devices were also tested for their sensitivity to different concentrations of heparin and found to exhibit a favorable increase in coagulation time for increasing heparin concentration.",
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Ramaswamy, B, Yeh, Y-T & Zheng, S 2011, Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969391, pp. 222-225, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 6/5/11. https://doi.org/10.1109/TRANSDUCERS.2011.5969391

Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood. / Ramaswamy, B.; Yeh, Yin-Ting; Zheng, Siyang.

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 222-225 5969391.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Ramaswamy B, Yeh Y-T, Zheng S. Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 222-225. 5969391 https://doi.org/10.1109/TRANSDUCERS.2011.5969391