Microfluidic inverse phase ELISA via manipulation of magnetic beads

Hong Chen, Assem Abolmatty, Mohammad Faghri

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid reagents to detect an antigenic analyte. These isolated but accessible plugs are preencapsulated in a microchannel by capillary force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (μIPELISA). μIPELISA has distinctive advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying, thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use μIPELISA to detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng μl-1 of digoxigenin- labeled amplicon, μIPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct μIPELISA in two parallel microchannels.

Original languageEnglish (US)
Pages (from-to)593-605
Number of pages13
JournalMicrofluidics and Nanofluidics
Volume10
Issue number3
DOIs
StatePublished - Mar 2011

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

Fingerprint

Dive into the research topics of 'Microfluidic inverse phase ELISA via manipulation of magnetic beads'. Together they form a unique fingerprint.

Cite this