TY - JOUR
T1 - Microfluidic multiple cross-correlated Coulter counter for improved particle size analysis
AU - Zhang, Wenchang
AU - Hu, Yuan
AU - Choi, Gihoon
AU - Liang, Shengfa
AU - Liu, Ming
AU - Guan, Weihua
N1 - Funding Information:
This project was partially supported by a grant from National Science Foundation, USA (ECCS-1710831)as well as China Postdoctoral Science Foundation (2017M620942), National Key R&D Program of China (2016YFB0402703)and National Key Scientific Instruments and Equipment Development Project of China (51727901).
Funding Information:
This project was partially supported by a grant from National Science Foundation, USA ( ECCS-1710831 ) as well as China Postdoctoral Science Foundation ( 2017M620942 ), National Key R&D Program of China ( 2016YFB0402703 ) and National Key Scientific Instruments and Equipment Development Project of China ( 51727901 ).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Coulter counters (a.k.a. resistive pulse sensors)were widely used to measure the size of biological cells and colloidal particles. One of the important parameters of Coulter counters is its size discriminative capability. This work reports a multiple pore-based microfluidic Coulter counter for improved size differentiation in a mixed sample. When a single particle translocated across an array of sensing pores, multiple time-related resistive pulse signals were generated. Due to the time correlation of these resistive pulse signals, we found a multiple cross-correlation analysis (MCCA)could enhance the sizing signal-to-noise (SNR)ratio by a factor of n1/2, where n is the pore numbers in series. This proof-of-concept is experimentally validated with polystyrene beads as well as human red blood cells. We anticipate this method would be highly beneficial for applications where improved size differentiation is required.
AB - Coulter counters (a.k.a. resistive pulse sensors)were widely used to measure the size of biological cells and colloidal particles. One of the important parameters of Coulter counters is its size discriminative capability. This work reports a multiple pore-based microfluidic Coulter counter for improved size differentiation in a mixed sample. When a single particle translocated across an array of sensing pores, multiple time-related resistive pulse signals were generated. Due to the time correlation of these resistive pulse signals, we found a multiple cross-correlation analysis (MCCA)could enhance the sizing signal-to-noise (SNR)ratio by a factor of n1/2, where n is the pore numbers in series. This proof-of-concept is experimentally validated with polystyrene beads as well as human red blood cells. We anticipate this method would be highly beneficial for applications where improved size differentiation is required.
UR - http://www.scopus.com/inward/record.url?scp=85066287785&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066287785&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.05.092
DO - 10.1016/j.snb.2019.05.092
M3 - Article
AN - SCOPUS:85066287785
VL - 296
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
M1 - 126615
ER -