Micropropagation of Hesperaloe parviflora

Arthur M. Richwine, Jimmy L. Tipton, Gary A. Thompson

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We successfully micropropagated Hesperaloe parviflora from mature plants. Shoot cultures were directly initiated from mature plants using pedicel bud explants on a modified Murashige and Skoog medium containing Nitsch and Nitseh vitamins and 1 μM zeatin riboside. Axillary shoot multiplication from established cultures was most responsive to changing concentrations of N6-benzyladenine (BA) with the greatest production on 6 μM BA. Growing shoots on a medium supplemented with 6 μM BA for 6 wk and then transferring cultures to a 1 μM BA medium for 6 more wk increased the number of transferable shoots, but not significantly. However, our data predicts that the maximum number of transferable shoots produced from a single microshoot would occur on media with 5.4 μM zeatin riboside. Shoots rooted easily in vitro or ex vitro and rooted shoots were easily acclimatized. The methods described in this paper are being used to commercially micropropagate H. parviflora.

Original languageEnglish (US)
Pages (from-to)262-266
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume32
Issue number4
DOIs
StatePublished - Jan 1 1996

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Plant Science

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