Microsatellite-centromere mapping in the zebrafish (danio rerio)

Elizabeth J. Kauffman, Erin E. Gestl, Don J. Kim, Charline Walker, Jill M. Hite, Guang Yan, Peter K. Rogan, Stephen L. Johnson, Keith Cheng

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Ten (CA)nmicrosatellite simple sequence repeat (SSR) markers, 1, 2, 12, 14, 16, 18, 20, 22, 26, and 29, were used to show high chiasma interference and to determine centromere-marker map distances in the zebrafish (Danio rerio). Of these, SSR 12 exhibited no recombinant tetratypes among 175 half-tetrad embryos, placing this marker within 1 cM of the centromere of Linkage Group XVII. Fractions of heterozygous half-tetrads for the remaining nine markers ranged from 0.64 to 0.89. Of these, six recombinant fractions were more than 0.67 (P< 0.05), indicating strong chiasma interference during female meiosis in the zebrafish. Consistent with previous mapping data, SSRs 2 and 20 of Linkage Group VI were tightly linked. Half-tetrad analysis will allow the mapping of the remaining centromeres and may be useful in the mapping of new genes and mutations in the zebrafish.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalGenomics
Volume30
Issue number2
DOIs
StatePublished - Jan 1 1995

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Centromere
Zebrafish
Microsatellite Repeats
Chromosome Mapping
Meiosis
Embryonic Structures
Mutation

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Kauffman, E. J., Gestl, E. E., Kim, D. J., Walker, C., Hite, J. M., Yan, G., ... Cheng, K. (1995). Microsatellite-centromere mapping in the zebrafish (danio rerio). Genomics, 30(2), 337-341. https://doi.org/10.1006/geno.1995.9869
Kauffman, Elizabeth J. ; Gestl, Erin E. ; Kim, Don J. ; Walker, Charline ; Hite, Jill M. ; Yan, Guang ; Rogan, Peter K. ; Johnson, Stephen L. ; Cheng, Keith. / Microsatellite-centromere mapping in the zebrafish (danio rerio). In: Genomics. 1995 ; Vol. 30, No. 2. pp. 337-341.
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Kauffman, EJ, Gestl, EE, Kim, DJ, Walker, C, Hite, JM, Yan, G, Rogan, PK, Johnson, SL & Cheng, K 1995, 'Microsatellite-centromere mapping in the zebrafish (danio rerio)', Genomics, vol. 30, no. 2, pp. 337-341. https://doi.org/10.1006/geno.1995.9869

Microsatellite-centromere mapping in the zebrafish (danio rerio). / Kauffman, Elizabeth J.; Gestl, Erin E.; Kim, Don J.; Walker, Charline; Hite, Jill M.; Yan, Guang; Rogan, Peter K.; Johnson, Stephen L.; Cheng, Keith.

In: Genomics, Vol. 30, No. 2, 01.01.1995, p. 337-341.

Research output: Contribution to journalArticle

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Kauffman EJ, Gestl EE, Kim DJ, Walker C, Hite JM, Yan G et al. Microsatellite-centromere mapping in the zebrafish (danio rerio). Genomics. 1995 Jan 1;30(2):337-341. https://doi.org/10.1006/geno.1995.9869