Microsatellite DNA loci for Western Hemlock [Tsuga heterophylla (Raf.) Sarg]

Vindhya Amarasinghe, Garth R. Brown, Judith E. Mank, John E. Carlson

Research output: Contribution to journalReview articlepeer-review

4 Scopus citations

Abstract

Polymorphic microsatellite loci were developed for Western Hemlock [Tsuga heterophylla (Raf.) Sarg], a prominent forest tree species in Western North America. Microsatellite-enriched libraries were screened for (CA)n dinucleotide repeats from which 33 positive clones were sequenced. Polymerase chain reaction (PCR) primers for 16 microsatellite loci were prepared and tested against DNA from unrelated Western Hemlock trees. The 12 most informative microsatellite loci are reported here. From four to 22 alleles per locus were observed, with an average expected heterozygousity of 0.799.

Original languageEnglish (US)
Pages (from-to)236-238
Number of pages3
JournalMolecular Ecology Notes
Volume2
Issue number3
DOIs
StatePublished - Sep 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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