Microsatellite DNA markers for assessing phylogeographic and population structure in Preble's meadow jumping mice (Zapus hudsonius preblei) and cross-amplification among neighbouring taxa

Timothy Lee King, Michael S. Eackles, Colleen C. Young

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We document the isolation and characterization of 14 tetranucleotide microsatellite DNA markers in Preble's meadow jumping mouse (Zapus hudsonius preblei). The identified markers displayed moderate levels of allelic diversity (averaging 4.9 alleles per locus) and heterozygosity (averaging 55.1%). Genotypic and allelic frequencies in a collection of 30 individuals conformed to Hardy-Weinberg equilibrium expectations and indicated no linkage disequilibrium. High levels of cross-amplification (95% overall) among neighbouring subspecies and two congeners (Zapus princeps and Zapus trinotatus) were observed. Multilocus genotypes resulting from these markers appear to provide ample genetic diversity for studies assessing individual- and population-level ecological interactions within Z. h. preblei and evolutionary relationships among neighbouring subspecies (Z. h. campestris, Z. h. intermedius, Z. h. pallidus and Z. h. luteus).

Original languageEnglish (US)
Pages (from-to)670-673
Number of pages4
JournalMolecular Ecology Notes
Volume6
Issue number3
DOIs
StatePublished - Sep 1 2006

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Linkage Disequilibrium
Genetic Markers
Microsatellite Repeats
meadow
Amplification
population structure
amplification
Alleles
Genotype
microsatellite repeats
DNA
subspecies
genetic markers
linkage disequilibrium
Population
gene frequency
heterozygosity
alleles
disequilibrium
genetic variation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "We document the isolation and characterization of 14 tetranucleotide microsatellite DNA markers in Preble's meadow jumping mouse (Zapus hudsonius preblei). The identified markers displayed moderate levels of allelic diversity (averaging 4.9 alleles per locus) and heterozygosity (averaging 55.1{\%}). Genotypic and allelic frequencies in a collection of 30 individuals conformed to Hardy-Weinberg equilibrium expectations and indicated no linkage disequilibrium. High levels of cross-amplification (95{\%} overall) among neighbouring subspecies and two congeners (Zapus princeps and Zapus trinotatus) were observed. Multilocus genotypes resulting from these markers appear to provide ample genetic diversity for studies assessing individual- and population-level ecological interactions within Z. h. preblei and evolutionary relationships among neighbouring subspecies (Z. h. campestris, Z. h. intermedius, Z. h. pallidus and Z. h. luteus).",
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Microsatellite DNA markers for assessing phylogeographic and population structure in Preble's meadow jumping mice (Zapus hudsonius preblei) and cross-amplification among neighbouring taxa. / King, Timothy Lee; Eackles, Michael S.; Young, Colleen C.

In: Molecular Ecology Notes, Vol. 6, No. 3, 01.09.2006, p. 670-673.

Research output: Contribution to journalArticle

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