Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland's warbler (Dendroica kirtlandii)

Tim L. King, Michael S. Eackles, Anne P. Henderson, Carol I. Bocetti, Dave Currie, Joseph M. Wunderle

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We document the isolation and characterization of 23 microsatellite DNA markers for the endangered Kirtland's warbler (Dendroica kirtlandii), a Nearctic/Neotropical migrant passerine. This suite of markers revealed moderate to high levels of allelic diversity (averaging 7.7 alleles per locus) and heterozygosity (averaging 72%). Genotypic frequencies at 22 of 23 (95%) markers conformed to Hardy-Weinberg equilibrium expectations, and no linkage disequilibrium was observed in blood samples taken from 14 warblers found on the wintering grounds in the Bahamas archipelago. Multilocus genotypes resulting from this suite of markers should reduce the amount of resources required for initiating new genetic studies assessing breeding structure, parentage, demographics, and individual-level ecological interactions for D. kirtlandii.

Original languageEnglish (US)
Pages (from-to)569-571
Number of pages3
JournalMolecular Ecology Notes
Volume5
Issue number3
DOIs
StatePublished - Sep 1 2005

Fingerprint

Songbirds
Bahamas
kinship
parentage
linkage disequilibrium
Genetic Markers
Microsatellite Repeats
population structure
heterozygosity
Blood
demographic statistics
microsatellite repeats
alleles
DNA
loci
genetic markers
genotype
blood
Linkage Disequilibrium
breeding

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

King, Tim L. ; Eackles, Michael S. ; Henderson, Anne P. ; Bocetti, Carol I. ; Currie, Dave ; Wunderle, Joseph M. / Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland's warbler (Dendroica kirtlandii). In: Molecular Ecology Notes. 2005 ; Vol. 5, No. 3. pp. 569-571.
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Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland's warbler (Dendroica kirtlandii). / King, Tim L.; Eackles, Michael S.; Henderson, Anne P.; Bocetti, Carol I.; Currie, Dave; Wunderle, Joseph M.

In: Molecular Ecology Notes, Vol. 5, No. 3, 01.09.2005, p. 569-571.

Research output: Contribution to journalArticle

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