Microtubule-assisted mechanism for functional metabolic macromolecular complex formation

Songon An, Yijun Deng, John W. Tomsho, Minjoung Kyoung, Stephen J. Benkovic

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Abstract

Evidence has been presented for a metabolic multienzyme complex, the purinosome, that participates in de novo purine biosynthesis to form clusters in the cytoplasm of living cells under purine-depleted conditions. Here we identified, using fluorescent live cell imaging, that a microtubule network appears to physically control the spatial distribution of purinosomes in the cytoplasm. Application of a cell-based assay measuring the rate of de novo purine biosynthesis confirmed that the metabolic activity of purinosomes was significantly suppressed in the absence of microtubules. Collectively, we propose a microtubule-assisted mechanism for functional purinosome formation in HeLa cells.

Original languageEnglish (US)
Pages (from-to)12872-12876
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number29
DOIs
Publication statusPublished - Jul 20 2010

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All Science Journal Classification (ASJC) codes

  • General

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