Microvascular anastomosis using a photochemical tissue bonding technique

Anne C. O'Neill, Jonathan M. Winograd, José L. Zeballos, Timothy Johnson, Mark A. Randolph, Kenneth E. Bujold, Irene E. Kochevar, Robert W. Redmond

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Background and Objectives: Photochemical tissue bonding (PTB) combines photoactive dyes with visible light to create fluid-tight seals between tissue surfaces without causing collateral thermal damage. The potential of PTB to improve outcomes over standard of care microsurgical reanastomoses of blood vessels in ex vivo and in vivo models was evaluated. Study Design: The mechanical strength and integrity of PTB and standard microsurgical suture repairs in ex vivo porcine brachial arteries (n = 10) were compared using hydrostatic testing of leak point pressure (LPP). Femoral artery repair in vivo was measured in Sprague-Dawley rats using either standard microvascular sutures (n = 20) or PTB (n = 20). Patency was evaluated at 6 hours (n = 10) and 8 weeks post-repair (n = 10) for each group. Results: PTB produced significantly higher LPPs (1,100 ± 150 mmHg) than suture repair (350 ± 40 mmHg, P < 0.001) in an ex vivo study. In an in vivo study all femoral arteries in both suture and PTB repair groups were patent at 6 hours post-repair. At 8 weeks post-repair the patency rate was 80% for both groups. No evidence of aneurysm formation was seen in either group and bleeding was absent from the repair site in the PTB-treated vessels, in contrast to the suture repair group. Conclusion: PTB is a feasible microvascular repair technique that results in an immediate, mechanically robust bond with short- and long-term patency rates equal to those for standard suture repair.

Original languageEnglish (US)
Pages (from-to)716-722
Number of pages7
JournalLasers in Surgery and Medicine
Volume39
Issue number9
DOIs
StatePublished - Oct 1 2007

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Sutures
Femoral Artery
Brachial Artery
Standard of Care
Aneurysm
Blood Vessels
Sprague Dawley Rats
Coloring Agents
Swine
Hot Temperature
Hemorrhage
Light
Pressure

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

O'Neill, A. C., Winograd, J. M., Zeballos, J. L., Johnson, T., Randolph, M. A., Bujold, K. E., ... Redmond, R. W. (2007). Microvascular anastomosis using a photochemical tissue bonding technique. Lasers in Surgery and Medicine, 39(9), 716-722. https://doi.org/10.1002/lsm.20548
O'Neill, Anne C. ; Winograd, Jonathan M. ; Zeballos, José L. ; Johnson, Timothy ; Randolph, Mark A. ; Bujold, Kenneth E. ; Kochevar, Irene E. ; Redmond, Robert W. / Microvascular anastomosis using a photochemical tissue bonding technique. In: Lasers in Surgery and Medicine. 2007 ; Vol. 39, No. 9. pp. 716-722.
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O'Neill, AC, Winograd, JM, Zeballos, JL, Johnson, T, Randolph, MA, Bujold, KE, Kochevar, IE & Redmond, RW 2007, 'Microvascular anastomosis using a photochemical tissue bonding technique', Lasers in Surgery and Medicine, vol. 39, no. 9, pp. 716-722. https://doi.org/10.1002/lsm.20548

Microvascular anastomosis using a photochemical tissue bonding technique. / O'Neill, Anne C.; Winograd, Jonathan M.; Zeballos, José L.; Johnson, Timothy; Randolph, Mark A.; Bujold, Kenneth E.; Kochevar, Irene E.; Redmond, Robert W.

In: Lasers in Surgery and Medicine, Vol. 39, No. 9, 01.10.2007, p. 716-722.

Research output: Contribution to journalArticle

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O'Neill AC, Winograd JM, Zeballos JL, Johnson T, Randolph MA, Bujold KE et al. Microvascular anastomosis using a photochemical tissue bonding technique. Lasers in Surgery and Medicine. 2007 Oct 1;39(9):716-722. https://doi.org/10.1002/lsm.20548